bioW

bioW
168

pimeloyl-CoA synthase (6-carboxyhexanoate-CoA ligase)

Locus
BSU_30240
Molecular weight
29.48 kDa
Isoelectric point
5.55
Protein length
259 aaSequenceBlast
Gene length
777 bpSequenceBlast
Function
biosynthesis of biotin
Product
pimeloyl-CoA synthase
Essential
no
E.C.
6.2.1.14
Synonyms
bioW
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1424 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,094,679 → 3,095,455
Phenotypes of a mutant
requires biotin for growth PubMed
The protein
Catalyzed reaction/ biological activity
ATP + CoA + heptanedioate --> AMP + diphosphate + heptanedioyl-CoA (according to UniProt)
Protein family
bioW family (single member, according to UniProt)
Structure
5FLG (PDB) PubMed
P53559 (AlphaFold)
Expression and Regulation
Operons
Genes
bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
Description
PubMed
Regulation
repressed in the presence of biotin (BirA) PubMed
Regulatory mechanism
BirA: repression, PubMed, in birA regulon
Open in new tab

bioW→ytbQ

2025-04-03 13:40:07

ghost

82

8e74711c48d33750c45114afa6e28dd08cdf4ea5

F7255B320407AE19B7B38F8E863B5EF4A9BF3D52

Biological materials
Mutant
GP4743 (trpC2 ΔbioW::neo), available in Jörg Stülke's lab
BKE30240 (ΔbioW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATTTCTCGCCCTTTCA,  downstream forward: _UP4_ATTTTAATAGAGTGGGAGGA
BKK30240 (ΔbioW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATTTCTCGCCCTTTCA,  downstream forward: _UP4_ATTTTAATAGAGTGGGAGGA
LacZ fusion
pBP627 (in pAC7), available in Fabian Commichau's lab
References
Reviews
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Original Publications
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C40116089BAD3F9831F8C837DC4F2E045753ABDE

Page visits: 3489

Time of last update: 2025-04-16 04:50:43

Author of last update: Robert.warneke