YkgB

• Description: 6-phosphogluconolactonase, general stress protein
  
  

Gene name	ykgB
Synonyms
Essential	no
Product	6-phosphogluconolactonase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: ykgB
MW, pI	38 kDa, 5.154
Gene length, protein length	1047 bp, 349 aa
Immediate neighbours	ykfD, ykgA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), carbon core metabolism

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU13010

Database entries

• BsubCyc: BSU13010

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 6-phosphogluconolactone --> 6-phosphogluconate (pentose phosphate pathway) PubMed

• Protein family: cycloisomerase 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU13010

• Structure:

• UniProt: O34499

• KEGG entry: [3]

• E.C. number: 3.1.1.31

Expression and regulation

• Operon: ykgA-ykgB PubMed

• Expression browser: ykgB PubMed

• Sigma factor: SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2628 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 7743 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1402 (spc, based on pGP1331), available in the Stülke lab

References

Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854] [WorldCat.org] [DOI] (I p)

Lynn C Thomason, Donald L Court, Atin R Datta, Rita Khanna, Judah L Rosner
Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase.
J Bacteriol: 2004, 186(24);8248-53
[PubMed:15576773] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)