yfiY
168

[wiki|ABC transporter for the siderophore schizokinen and arthrobactin (binding protein), works with ATPase YusV

Locus

BSU_08440

Molecular weight

36.15 kDa

Isoelectric point

7.17

Protein length

325 aaSequenceBlast

Gene length

978 bpSequenceBlast

Function

acquisition of iron

Product

ABC transporter for the siderophore schizokinen and arthrobactin (binding protein)

Essential

Synonyms

yfiY

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0614 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

919,366 920,343

Phenotypes of a mutant

poor growth with the xenosiderophores arthrobactin or aerobactin as single source of iron PubMed

The protein

Protein family

Bacterial solute-binding protein 8 family (according to UniProt)

Domains

Fe/B12 periplasmic-binding domain (aa 56-325) (according to UniProt)

Structure

3TNY (PDB) (from B. cereus, 57% identity)

O31567 (AlphaFold)

Modification

phosphorylation on (Ser-290 OR Thr-302) PubMed

Localization

associated to the membrane (via YfhA-YfiZ) PubMed

extracellular (signal peptide) PubMed

Expression and Regulation

Operons

Genes

yfiY

Description

PubMed

Regulation

repressed unless the cells enter an iron starvation (Fur) PubMed

Regulatory mechanism

Fur: repression, PubMed, in fur regulon

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Biological materials

Mutant

MGNA-C311 (yfiY::erm), available at the NBRP B. subtilis, Japan

BKE08440 (yfiY::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTCCTCCCAATAT, downstream forward: _UP4_AAATAAAAAAAGACTCCGTC

BKK08440 (yfiY::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTTTCCTCCCAATAT, downstream forward: _UP4_AAATAAAAAAAGACTCCGTC

References

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Time of last update: 2025-03-31 18:44:25

Author of last update: Jstuelk

yfiY 168

yfiY
168