YvcK

• Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
  
  

Gene name	yvcK
Synonyms
Essential	no
Product	unknown
Function	localization of PBP1
Gene expression levels in SubtiExpress: yvcK
MW, pI	34 kDa, 5.408
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	yvcL, yvcJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, cell shape

This gene is a member of the following regulons

Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU34760

Phenotypes of a mutant

• unable to grow with gluconeogenic substrates as single carbon source PubMed
• filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed

Database entries

• BsubCyc: BSU34760

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0052 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: localized as a helical-like pattern PubMed

Database entries

• BsubCyc: BSU34760

• Structure:

• UniProt: O06974

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Expression browser: yvcK PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 96 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 246 PubMed

Biological materials

• Mutant:

• Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage

Anne Galinier, University of Marseille, France

References

Reviews

PubMed