IspH

• Description: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	ispH
Synonyms	yqfP
Essential	yes PubMed
Product	(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Function	MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispH
Metabolic function and regulation of this protein in SubtiPathways: IspH
MW, pI	34 kDa, 5.615
Gene length, protein length	942 bp, 314 aa
Immediate neighbours	yqfQ, yqfO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes

The gene

Basic information

• Locus tag: BSU25160

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25160

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Isopentenyl diphosphate + NAD(P)⁺ + H₂O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H (according to Swiss-Prot)

• Protein family: ispH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU25160

• Structure: 3F7T (IspH from E. coli, 38% identity) PubMed

• UniProt: P54473

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ispH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 175 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 433 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: