hemB

hemB
168

porphobilinogen synthase

Locus
BSU_28130
Molecular weight
36.05 kDa
Isoelectric point
4.98
Protein length
324 aaSequenceBlast
Gene length
975 bpSequenceBlast
Function
heme biosynthesis
Product
porphobilinogen synthase
Essential
no
E.C.
4.2.1.24
Synonyms
hemB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0113 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,874,202  2,875,176
The protein
Catalyzed reaction/ biological activity
2 5-aminolevulinate --> porphobilinogen + 2 H2O (according to UniProt)
Protein family
ALAD family (single member, according to UniProt)
Cofactors
zinc
Structure
1W5Q (PDB) (from Pseudomonas aeruginosa, 48% identity) PubMed
P30950 (AlphaFold)
Modification
phosphorylated on Arg-217 PubMed
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
hemA-hemX-hemC-hemD-hemB-hemL
Description
PubMed
Regulation
induced by hydrogen peroxide (PerR) PubMed
Regulatory mechanism
PerR: repression, PubMed, in perR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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hemA→hemL

2025-03-29 16:29:02

ghost

108

64840dd729762069005c4bd541ed0ddec6e74ace

168BC8B91CBFE3EA050DA8A9187C8C67D6BBE4F9

Biological materials
Mutant
BKE28130 (hemB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCGGTGTCTATTAAATGATT,  downstream forward: _UP4_TAATTTTATTCAGTTGACAG
BKK28130 (hemB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCGGTGTCTATTAAATGATT,  downstream forward: _UP4_TAATTTTATTCAGTTGACAG
References
Reviews
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Original Publications
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70263A55987F110209367CF45AD65A584AD3077D

Page visits: 3320

Time of last update: 2025-04-16 01:06:44

Author of last update: Melvin.boenninger