Difference between revisions of "ParB"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 341 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 362 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:27, 17 April 2014

  • Description: chromosome positioning near the pole and transport through the polar septum / antagonist of Soj-dependent inhibition of sporulation initiation

Gene name spo0J
Synonyms parB, spo0JB
Essential no
Product site-specific DNA-binding protein
Function chromosome positioning before asymmetric septation,

specifying its orientation, and imposing directionality on

its subsequent transport through the septum; required for sporulation

(antagonist of Soj-dependent inhibition of sporulation initiation)

Gene expression levels in SubtiExpress: spo0J
Interactions involving this protein in SubtInteract: Spo0J
MW, pI 32 kDa, 8.417
Gene length, protein length 846 bp, 282 aa
Immediate neighbours yyaC, soj
Sequences Protein DNA DNA_with_flanks
Genetic context
Spo0J context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Spo0J expression.png















Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

  • Locus tag: BSU40960

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • inhibits Soj dimerization and concomitant DNA-binding activity by stimulating Soj ATPase activity PubMed
    • recruits the condensin complex to the DNA origin region PubMed
  • Protein family: parB family (according to Swiss-Prot)
  • Paralogous protein(s): Noc

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 341 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 362 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)

Original publications