rpmD

rpmD
168

ribosomal protein uL30

Locus
BSU_01340
Molecular weight
6.50 kDa
Isoelectric point
10.8
Protein length
59 aaSequenceBlast
Gene length
180 bpSequenceBlast
Function
translation
Product
ribosomal protein L30 (uL30)
Essential
yes
Synonyms
rpmD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1841 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, Stringent response

Gene
Coordinates
143,875  144,054
Phenotypes of a mutant
essential PubMed
The protein
Protein family
universal ribosomal protein uL30 family (single member, according to UniProt)
Structure
3J9W (PDB) (the ribosome) PubMed
P19947 (AlphaFold)
Additional information
this ribosomal protein is lacking in some bacteria PubMed
Expression and Regulation
Operons
Genes
rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
Description
PubMed
Regulation
strongly repressed in response to glucose starvation in M9 medium PubMed
likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon
Additional information
term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
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rpsJ→rplQ

2025-04-02 13:05:40

Jstuelk

147

911D53E804EEFE6335D6A0F79722F9870F387CD3

9027854E648E8BF3E4A3C8492D350C69AC71C218

References
Galperin MY, Wolf YI, Garushyants SK, Vera Alvarez R, Koonin EVNon-essential ribosomal proteins in bacteria and archaea identified using COGs.Journal of bacteriology. 2021 Mar 22; . PMID: 33753464
Sohmen D, Chiba S, Shimokawa-Chiba N, Innis CA, Berninghausen O, Beckmann R, Ito K, Wilson DN Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling. Nature communications. 2015 Apr 23; 6:6941. doi:10.1038/ncomms7941. PMID:25903689
Akanuma G, Nanamiya H, Natori Y, Yano K, Suzuki S, Omata S, Ishizuka M, Sekine Y, Kawamura F Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. Journal of bacteriology. 2012 Nov; 194(22):6282-91. doi:10.1128/JB.01544-12. PMID:23002217
Lauber MA, Running WE, Reilly JP B. subtilis ribosomal proteins: structural homology and post-translational modifications. Journal of proteome research. 2009 Sep; 8(9):4193-206. doi:10.1021/pr801114k. PMID:19653700
Eymann C, Homuth G, Scharf C, Hecker M Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis. Journal of bacteriology. 2002 May; 184(9):2500-20. . PMID:11948165
Li X, Lindahl L, Sha Y, Zengel JM Analysis of the Bacillus subtilis S10 ribosomal protein gene cluster identifies two promoters that may be responsible for transcription of the entire 15-kilobase S10-spc-alpha cluster. Journal of bacteriology. 1997 Nov; 179(22):7046-54. . PMID:9371452
Suh JW, Boylan SA, Oh SH, Price CW Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region. Gene. 1996 Feb 22; 169(1):17-23. . PMID:8635744

87741B91E3081B7CC1C8AF275B38A52C066F018C

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Time of last update: 2025-04-04 03:16:45

Author of last update: Jstuelk