Difference between revisions of "YkwC"

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* '''Additional information:'''
 
* '''Additional information:'''
 
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 7715 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 3613 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:24, 17 April 2014

  • Description: putative beta-hydroxyacid dehydrogenase

Gene name ykwC
Synonyms
Essential no
Product putative beta-hydroxyacid dehydrogenase
Function unknown
Gene expression levels in SubtiExpress: ykwC
MW, pI 30 kDa, 5.109
Gene length, protein length 864 bp, 288 aa
Immediate neighbours mcpC, ykwD
Sequences Protein DNA DNA_with_flanks
Genetic context
YkwC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkwC expression.png















Categories containing this gene/protein

poorly characterized/ putative enzymes, most abundant proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU13960

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: 3-hydroxyisobutyrate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s): YfjR

Extended information on the protein

  • Kinetic information:
  • Modification: phosphorylated on Ser-281 by PrkC, dephosphorylated by PrpC, phosphorylation abolishes the oxidoreductase activity of YkwC in vitro and in vivo PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 7715 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3613 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Vaishnavi Ravikumar, Lei Shi, Karsten Krug, Abderahmane Derouiche, Carsten Jers, Charlotte Cousin, Ahasanul Kobir, Ivan Mijakovic, Boris Macek
Quantitative phosphoproteome analysis of Bacillus subtilis reveals novel substrates of the kinase PrkC and phosphatase PrpC.
Mol Cell Proteomics: 2014, 13(8);1965-78
[PubMed:24390483] [WorldCat.org] [DOI] (I p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)