YxxG

• Description: immunity protein, protects the cell against the toxic activity of WapA
  
  

Gene name	yxxG
Synonyms	wapI
Essential	no
Product	immunity protein
Function	intercellular competition
Gene expression levels in SubtiExpress: yxxG
Interactions involving this protein in SubtInteract: YxxG
MW, pI	16 kDa, 4.424
Gene length, protein length	426 bp, 142 aa
Immediate neighbours	yxiF, wapA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, phosphoproteins,

This gene is a member of the following regulons

DegU regulon, WalR regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU39220

Phenotypes of a mutant

• severe growth defect PubMed

Database entries

• BsubCyc: BSU39220

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • YxxG inhibits the toxic activity of WapA PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Tyr-102 OR Ser-105) PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • WapA-YxxG PubMed

• Localization:

Database entries

• BsubCyc: BSU39220

• Structure:

• UniProt: Q07836

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: wapA-yxxG PubMed

• Expression browser: yxxG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed at high salt concentration (DegU-P) PubMed

• Regulatory mechanism:
  • DegU-P: transcription repression PubMed
  • activated by YvrHb PubMed
  • WalR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2912 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1271 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sanna Koskiniemi, James G Lamoureux, Kiel C Nikolakakis, Claire t'Kint de Roodenbeke, Michael D Kaplan, David A Low, Christopher S Hayes
Rhs proteins from diverse bacteria mediate intercellular competition.
Proc Natl Acad Sci U S A: 2013, 110(17);7032-7
[PubMed:23572593] [WorldCat.org] [DOI] (I p)

Letal I Salzberg, Leagh Powell, Karsten Hokamp, Eric Botella, David Noone, Kevin M Devine
The WalRK (YycFG) and σ(I) RsgI regulators cooperate to control CwlO and LytE expression in exponentially growing and stressed Bacillus subtilis cells.
Mol Microbiol: 2013, 87(1);180-95
[PubMed:23199363] [WorldCat.org] [DOI] (I p)

Taryn B Kiley, Nicola R Stanley-Wall
Post-translational control of Bacillus subtilis biofilm formation mediated by tyrosine phosphorylation.
Mol Microbiol: 2010, 78(4);947-63
[PubMed:20815827] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

Masakuni Serizawa, Keisuke Kodama, Hiroki Yamamoto, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses.
Biosci Biotechnol Biochem: 2005, 69(11);2155-69
[PubMed:16306698] [WorldCat.org] [DOI] (P p)

V Dartois, M Débarbouillé, F Kunst, G Rapoport
Characterization of a novel member of the DegS-DegU regulon affected by salt stress in Bacillus subtilis.
J Bacteriol: 1998, 180(7);1855-61
[PubMed:9537385] [WorldCat.org] [DOI] (P p)