Difference between revisions of "AlsS"

From SubtiWiki
Jump to: navigation, search
Line 37: Line 37:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
+
<br/><br/>
<br/><br/><br/><br/><br/><br/>
 
  
  
Line 81: Line 80:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
Line 148: Line 147:
  
 
=References=
 
=References=
'''Additional publications:'''  {{PubMed|22178965}}
+
<pubmed>19087206,10986270, 20081037,16428414, 19383131, 7685336, 16428414  19684168 24734205 22178965</pubmed>
<pubmed>19087206,10986270, 20081037,16428414, 19383131, 7685336, 16428414  19684168 </pubmed>
 
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:29, 16 April 2014

  • Description: acetolactate synthase

Gene name alsS
Synonyms
Essential no
Product acetolactate synthase
Function overflow metabolism
Gene expression levels in SubtiExpress: alsS
Metabolic function and regulation of this protein in SubtiPathways:
alsS
MW, pI 61 kDa, 5.164
Gene length, protein length 1713 bp, 571 aa
Immediate neighbours alsD, alsR
Sequences Protein DNA DNA_with_flanks
Genetic context
AlsS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AlsS2 expression.png
















Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

AlsR regulon, Rex regulon, stringent response

The gene

Basic information

  • Locus tag: BSU36010

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
  • Protein family: TPP enzyme family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by acetate (AlsR) PubMed
    • repressed as long as terminal electron acceptors are available for respiration (Rex) PubMed
    • subject to positive stringent control upon lysine starvation PubMed

Note: since acetate formation requires ackA activation by CcpA there is an indirect effect of CcpA on the alsSD operon: the operon is not expressed in ccpA mutants

  • Regulatory mechanism:
    • stringent response: due to presence of adenines at +1 and +2 positions of the transcript PubMed
    • AlsR: transcription activation in the presence of acetate PubMed
    • Rex: transcription repression if the ratio NADH2/NAD is high PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References