putP

putP
168

high affinity proline permease

Locus
BSU_03220
Molecular weight
48.25 kDa
Isoelectric point
8.62
Protein length
473 aaSequenceBlast
Gene length
1422 bpSequenceBlast
Function
proline uptake
Product
high affinity proline permease
Essential
no
Synonyms
putP, ycgO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0591 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CodY regulon, Spo0A regulon, PutR regulon

Gene
Coordinates
347,150 → 348,571
Phenotypes of a mutant
no growth with proline as single source of carbon or nitrogen PubMed
The protein
Catalyzed reaction/ biological activity
uptake of proline coupled to the uptake of sodium ions
Protein family
sodium:solute symporter (SSF) (TC 2.A.21) family (according to UniProt)
Structure
2XQ2 (PDB) (from Vibrio parahaemolyticus, 26% identity) PubMed
P94392 (AlphaFold)
Paralogous protein(s)
OpuE
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
putP
Description
(internal promoter) PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the mRNA is cleaved by RNase III PubMed
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putP

2025-03-08 01:58:28

Jstuelk

66

ba423fcf0254111de91945f6c4e9adef9d3e72a3

08A76DA5A75B56B9BB4705D2C33B96AA0C63A5C3

Genes
putB-putC-putP
Description
PubMed
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
PutR: activation, PubMed, in putR regulon
Spo0A: activation, PubMed, in spo0A regulon
CodY: repression, displacement of PutR PubMed, in codY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
overexpressed in a pnpA mutant PubMed
the putP part of the mRNA is cleaved by RNase III PubMed
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putB→putP

2025-04-04 01:57:06

Jstuelk

117

d991744c53f7fc7228712ba483de8c17cc05c88a

72CAEF916BFA077D3E9623FC66EECF026978B0D8

Biological materials
Mutant
MGNA-B994 (ycgO::erm), available at the NBRP B. subtilis, Japan
available in Erhard Bremer's lab PubMed
BKE03220 (ΔputP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCCGCCATATAAATAC,  downstream forward: _UP4_TAAAGATCGAAAGGAGGAGG
BKK03220 (ΔputP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCCGCCATATAAATAC,  downstream forward: _UP4_TAAAGATCGAAAGGAGGAGG
References
DiChiara JM, Liu B, Figaro S, Condon C, Bechhofer DH Mapping of internal monophosphate 5' ends of Bacillus subtilis messenger RNAs and ribosomal RNAs in wild-type and ribonuclease-mutant strains. Nucleic acids research. 2016 Apr 20; 44(7):3373-89. doi:10.1093/nar/gkw073. PMID:26883633
Bracher S, Guérin K, Polyhach Y, Jeschke G, Dittmer S, Frey S, Böhm M, Jung H Glu-311 in External Loop 4 of the Sodium/Proline Transporter PutP Is Crucial for External Gate Closure. The Journal of biological chemistry. 2016 Mar 04; 291(10):4998-5008. doi:10.1074/jbc.M115.675306. PMID:26728461
Zaprasis A, Hoffmann T, Stannek L, Gunka K, Commichau FM, Bremer E The γ-aminobutyrate permease GabP serves as the third proline transporter of Bacillus subtilis. Journal of bacteriology. 2014 Feb; 196(3):515-26. doi:10.1128/JB.01128-13. PMID:24142252
Moses S, Sinner T, Zaprasis A, Stöveken N, Hoffmann T, Belitsky BR, Sonenshein AL, Bremer E Proline utilization by Bacillus subtilis: uptake and catabolism. Journal of bacteriology. 2012 Feb; 194(4):745-58. doi:10.1128/JB.06380-11. PMID:22139509
Huang SC, Lin TH, Shaw GC PrcR, a PucR-type transcriptional activator, is essential for proline utilization and mediates proline-responsive expression of the proline utilization operon putBCP in Bacillus subtilis. Microbiology (Reading, England). 2011 Dec; 157(Pt 12):3370-7. doi:10.1099/mic.0.054197-0. PMID:21964733
Belitsky BR Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon. Journal of molecular biology. 2011 Oct 21; 413(2):321-36. doi:10.1016/j.jmb.2011.08.003. PMID:21840319
Watanabe A, Choe S, Chaptal V, Rosenberg JM, Wright EM, Grabe M, Abramson J The mechanism of sodium and substrate release from the binding pocket of vSGLT. Nature. 2010 Dec 16; 468(7326):988-91. doi:10.1038/nature09580. PMID:21131949

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Page visits: 6979

Time of last update: 2025-04-06 07:56:54

Author of last update: Melvin.boenninger