SteT

• Description: serine/ threonine exchanger transporter
  
  

Gene name	steT
Synonyms	ykbA
Essential	no
Product	serine/ threonine exchanger transporter
Function	exchange of serine and threonine
Gene expression levels in SubtiExpress: steT
Metabolic function and regulation of this protein in SubtiPathways: SteT
MW, pI	46 kDa, 9.184
Gene length, protein length	1314 bp, 438 aa
Immediate neighbours	ykaA, mhqA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, membrane proteins

The gene

Basic information

• Locus tag: BSU12860

Database entries

• BsubCyc: BSU12860

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:
  • amino acid, polyamine, organocation (APC) superfamily of transporters, TC 2.A.3

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane

Database entries

• BsubCyc: BSU12860

• Structure:

• UniProt: O34739

• KEGG entry: [2]

• T.C. number: 2.A.3.8.12

Expression and regulation

• Operon: steT

• Expression browser: steT PubMed

• Sigma factor:

• Regulation: expression activated by glucose (4.2 fold) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Paola Bartoccioni, César Del Rio, Merce Ratera, Lukasz Kowalczyk, Jocelyn M Baldwin, Antonio Zorzano, Matthias Quick, Stephen A Baldwin, José Luis Vázquez-Ibar, Manuel Palacín
Role of transmembrane domain 8 in substrate selectivity and translocation of SteT, a member of the L-amino acid transporter (LAT) family.
J Biol Chem: 2010, 285(37);28764-76
[PubMed:20610400] [WorldCat.org] [DOI] (I p)

Christian A Bippes, Antra Zeltina, Fabio Casagrande, Merce Ratera, Manuel Palacin, Daniel J Muller, Dimitrios Fotiadis
Substrate binding tunes conformational flexibility and kinetic stability of an amino acid antiporter.
J Biol Chem: 2009, 284(28);18651-63
[PubMed:19419962] [WorldCat.org] [DOI] (P p)

Núria Reig, César del Rio, Fabio Casagrande, Mercè Ratera, Josep Lluís Gelpí, David Torrents, Peter J F Henderson, Hao Xie, Stephen A Baldwin, Antonio Zorzano, Dimitrios Fotiadis, Manuel Palacín
Functional and structural characterization of the first prokaryotic member of the L-amino acid transporter (LAT) family: a model for APC transporters.
J Biol Chem: 2007, 282(18);13270-81
[PubMed:17344220] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)