walJ

walJ
168

5'-3' double-stranded DNA exonuclease, coordination of cell division with DNA replication

Locus
BSU_40370
Molecular weight
29.77 kDa
Isoelectric point
5.5
Protein length
264 aaSequenceBlast
Gene length
795 bpSequenceBlast
Function
coordination of cell division with DNA replication
Product
5'-3' double-stranded DNA exonuclease
Essential
no
E.C.
3.-.-.-
Synonyms
walJ, yycJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1235 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,148,851 → 4,149,645
Phenotypes of a mutant
the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited PubMed
increased sensitivity to a narrow spectrum of cephalosporin antibiotics PubMed
disruption of the corresponding gene in B. anthracis results in a mutator phenotype PubMed
improved survival after DNA replication arrest imposed by inhibition of PolC activity PubMed
The protein
Catalyzed reaction/ biological activity
5'-3' exonuclease activity at the 5' termini and at nicks of double-stranded DNA PubMed
the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of cell division with DNA replication PubMed
Protein family
predicted member of the metallo-β-lactamase superfamily
Cofactors
Mn(II) PubMed
Structure
C0SP91 (AlphaFold)
Expression and Regulation
Operons
Genes
walR-walK-walH-walI-walJ-htrC
Description
PubMed
Regulation
htrC transcript expressed during sporulation PubMed
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walR→htrC

2025-04-04 01:28:33

ghost

163

4e891bb3dceb8eda2e5ad4f9ed646a611afbe349

32BB8D419E54714C8925B07FBBCF691251CA4145

Biological materials
Mutant
GP3558 (ΔwalJ::aphA3), available in Jörg Stülke's lab
MGNA-B824 (yycJ::erm), available at the NBRP B. subtilis, Japan
1A1044 ( walJ::spec), PubMed, available at [BGhttp://bgsc.org/getdetail.php?bgscid=1A1044]
BKE40370 (ΔwalJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTGTCTCACTCCATT,  downstream forward: _UP4_TAATTTTTCTATGTAAATCA
BKK40370 (ΔwalJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTGTCTCACTCCATT,  downstream forward: _UP4_TAATTTTTCTATGTAAATCA
References
Reviews
Lenhart JS, Pillon MC, Guarné A, Biteen JS, Simmons LA Mismatch repair in Gram-positive bacteria. Research in microbiology. 2016 Jan; 167(1):4-12. doi:10.1016/j.resmic.2015.08.006. pii:S0923-2508(15)00146-1. PMID:26343983
Original Publications
Schons-Fonseca L, Lazova MD, Smith JL, Anderson ME, Grossman ADBeneficial and detrimental genes in the cellular response to replication arrest.PLoS genetics. 2022 Dec 27; 18(12):e1010564. PMID: 36574412
Yang H, Yung M, Li L, Hoch JA, Ryan CM, Kar UK, Souda P, Whitelegge JP, Miller JH Evidence that YycJ is a novel 5'-3' double-stranded DNA exonuclease acting in Bacillus anthracis mismatch repair. DNA repair. 2013 May 01; 12(5):334-46. doi:10.1016/j.dnarep.2013.02.002. pii:S1568-7864(13)00042-6. PMID:23491602
Yang H, Sikavi C, Tran K, McGillivray SM, Nizet V, Yung M, Chang A, Miller JH Papillation in Bacillus anthracis colonies: a tool for finding new mutators. Molecular microbiology. 2011 Mar; 79(5):1276-93. doi:10.1111/j.1365-2958.2011.07519.x. PMID:21205011
Biller SJ, Wayne KJ, Winkler ME, Burkholder WF The putative hydrolase YycJ (WalJ) affects the coordination of cell division with DNA replication in Bacillus subtilis and may play a conserved role in cell wall metabolism. Journal of bacteriology. 2011 Feb; 193(4):896-908. doi:10.1128/JB.00594-10. PMID:21169496
Fabret C, Hoch JA A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy. Journal of bacteriology. 1998 Dec; 180(23):6375-83. . PMID:9829949

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Time of last update: 2025-04-04 10:09:34

Author of last update: Jstuelk