Fbp

• Description: fructose-1,6-bisphosphatase, involved in gluconeogenesis
  
  

Gene name	fbp
Synonyms	fdp, yydE
Essential	no
Product	fructose-1,6-bisphosphatase
Function	gluconeogenesis
Gene expression levels in SubtiExpress: fbp
Metabolic function and regulation of this protein in SubtiPathways: fbp
MW, pI	77 kDa, 5.601
Gene length, protein length	2013 bp, 671 aa
Immediate neighbours	yydF, yydD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism

The gene

Basic information

• Locus tag: BSU40190

Database entries

• BsubCyc: BSU40190

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate + H₂O = D-fructose 6-phosphate + phosphate (according to Swiss-Prot)

• Protein family: FBPase class 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • specific activity: 0.203 µmol min^-1 (mg protein)^-1 PubMed

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU40190

• Structure:

• UniProt: Q45597

• KEGG entry: [3]

• E.C. number: 3.1.3.11

Expression and regulation

• Operon: fpb PubMed

• Expression browser: fbp PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Michael Kohlstedt, Praveen K Sappa, Hanna Meyer, Sandra Maaß, Adrienne Zaprasis, Tamara Hoffmann, Judith Becker, Leif Steil, Michael Hecker, Jan Maarten van Dijl, Michael Lalk, Ulrike Mäder, Jörg Stülke, Erhard Bremer, Uwe Völker, Christoph Wittmann
Adaptation of Bacillus subtilis carbon core metabolism to simultaneous nutrient limitation and osmotic challenge: a multi-omics perspective.
Environ Microbiol: 2014, 16(6);1898-917
[PubMed:24571712] [WorldCat.org] [DOI] (I p)

Y Fujita, K Yoshida, Y Miwa, N Yanai, E Nagakawa, Y Kasahara
Identification and expression of the Bacillus subtilis fructose-1, 6-bisphosphatase gene (fbp).
J Bacteriol: 1998, 180(16);4309-13
[PubMed:9696785] [WorldCat.org] [DOI] (P p)

Y Fujita, E Freese
Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase.
J Bacteriol: 1981, 145(2);760-7
[PubMed:6257649] [WorldCat.org] [DOI] (P p)

Y Fujita, E Freese
Purification and properties of fructose-1,6-bisphosphatase of Bacillus subtilis.
J Biol Chem: 1979, 254(12);5340-9
[PubMed:221467] [WorldCat.org] (P p)