rlmQ

rlmQ
168

23S rRNA methyltransferase, methylates an G in the A-site of the peptidyl transferase center

Locus
BSU_38360
Molecular weight
44.28 kDa
Isoelectric point
9.18
Protein length
396 aaSequenceBlast
Gene length
1191 bpSequenceBlast
Function
rRNA modification
Product
23S rRNA methyltransferase
Essential
no
Synonyms
ywbD, ipa-19d
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1092 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,935,824 → 3,937,014
The protein
Catalyzed reaction/ biological activity
formation of 7-methylguanosine (m7G) at position 2574 of the 23S rRNA PubMed
Protein family
Methyltransferase superfamily (according to UniProt)
Domains
PUA domain (aa 1-79) (according to UniProt)
Structure
3VSE (PDB) (from Staphylococcus aureus, 48% identity) PubMed
P39587 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
rlmQ
Description
PubMed
Regulatory mechanism
SinR: repression, PubMed, in sinR regulon
Open in new tab

rlmQ

2025-03-22 11:30:27

Bzhu

75

5131cb21f8d82dbb7973c86d47ac11a401417729

6B17BA667ACF2A3CC648806DD337B723268986BA

Biological materials
Mutant
MGNA-B222 (ywbD::erm), available at the NBRP B. subtilis, Japan
BKE38360 (ΔrlmQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATGTCGTCCTCTTTC,  downstream forward: _UP4_TAAATGAAAAAGCTGCCCTG
BKK38360 (ΔrlmQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATGTCGTCCTCTTTC,  downstream forward: _UP4_TAAATGAAAAAGCTGCCCTG
LacZ fusion
GP1614 amyE::p(ywbD-lacZ cat), constructed with pGP2150 based on pAC5, available in Jörg Stülke's lab
References
Loading

F2F0B16E5F55C8C91C20E4FFC7A63E3CD09D4EFD

Page visits: 3100

Time of last update: 2025-04-12 10:56:11

Author of last update: Jstuelk