ywjA

ywjA
168

similar to ABC transporter (ATP-binding protein)

Locus
BSU_37230
Molecular weight
64.39 kDa
Isoelectric point
6.14
Protein length
575 aaSequenceBlast
Gene length
1728 bpSequenceBlast
Function
unknown
Product
ABC transporter (ATP-binding protein)
Essential
no
Synonyms
ywjA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1132 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,819,754 → 3,821,481
The protein
Protein family
ABC transporter superfamily (according to UniProt)
Domains
has both a membrane-spanning and an ATP-binding domain PubMed
ABC transmembrane type-1 domain (aa 16-299) (according to UniProt)
ABC transporter domain (aa 333-567) (according to UniProt)
Structure
2HYD (PDB) (from Staphylococcus aureus, 38% identity) PubMed
P45861 (AlphaFold)
Paralogous protein(s)
YfiC, BmrA, YknU, YknV, YgaD, BmrC, YfiB
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
ywiE-ywjA-ywjB
Description
PubMed
Regulatory mechanism
Fur: repression, PubMed, in fur regulon
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
Open in new tab

ywiE->ywjB

2025-04-05 22:56:46

Bzhu

68

212b18f4698de19cc17ce4d25e56c42b2e6ee3aa

1388A2364B0BC385CFA90298AFC1348C8DFABFBB

Genes
ywjA-ywjB
Description
PubMed
Open in new tab

ywjA->ywjB

2025-04-05 23:37:15

Bzhu

72

6eff6d033346d8ef043489abb2fed27db7b585c7

6CEEBC9D0EB07CCE364936AB1A948F2DA1B1005D

Biological materials
Mutant
MGNA-B662 (ywjA::erm), available at the NBRP B. subtilis, Japan
BKE37230 (ΔywjA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCTATCTTCCCCCAATG,  downstream forward: _UP4_ATGGTGCATCGATAGGAGGA
BKK37230 (ΔywjA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCTATCTTCCCCCAATG,  downstream forward: _UP4_ATGGTGCATCGATAGGAGGA
References
Mahendran A, Orlando BJGenome wide structural prediction of ABC transporter systems in Bacillus subtilis.Frontiers in microbiology. 2024; 15:1469915. PMID: 39397791
Arrieta-Ortiz ML, Hafemeister C, Bate AR, Chu T, Greenfield A, Shuster B, Barry SN, Gallitto M, Liu B, Kacmarczyk T, Santoriello F, Chen J, Rodrigues CD, Sato T, Rudner DZ, Driks A, Bonneau R, Eichenberger P An experimentally supported model of the Bacillus subtilis global transcriptional regulatory network. Molecular systems biology. 2015 Nov 17; 11(11):839. doi:10.15252/msb.20156236. PMID:26577401
Dawson RJ, Locher KP Structure of a bacterial multidrug ABC transporter. Nature. 2006 Sep 14; 443(7108):180-5. . PMID:16943773
Baichoo N, Wang T, Ye R, Helmann JD Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon. Molecular microbiology. 2002 Sep; 45(6):1613-29. . PMID:12354229
Quentin Y, Fichant G, Denizot F Inventory, assembly and analysis of Bacillus subtilis ABC transport systems. Journal of molecular biology. 1999 Apr 02; 287(3):467-84. . PMID:10092453

D5A3C316BD4567A0155F70330E32ACDE18F063FE

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Time of last update: 2025-04-06 03:42:42

Author of last update: Jstuelk