LytB

• Description: modifier protein of major autolysin LytC
  
  

Gene name	lytB
Synonyms	cwbA
Essential	no
Product	modifier protein of major autolysin LytC
Function	autolysis
Gene expression levels in SubtiExpress: lytB
MW, pI	76 kDa, 9.835
Gene length, protein length	2115 bp, 705 aa
Immediate neighbours	lytC, lytA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, membrane proteins

This gene is a member of the following regulons

SigD regulon, SlrR regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU35630

Database entries

• BsubCyc: BSU35630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35630

• Structure:

• UniProt: Q02113

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lytA-lytB-lytC PubMed

• Expression browser: lytB PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation:

• Regulatory mechanism:
  • YvrHb: transcription repression PubMed
  • SlrR (in complex with SinR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A789 ( lytB::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yunrong Chai, Thomas Norman, Roberto Kolter, Richard Losick
An epigenetic switch governing daughter cell separation in Bacillus subtilis.
Genes Dev: 2010, 24(8);754-65
[PubMed:20351052] [WorldCat.org] [DOI] (I p)

Masakuni Serizawa, Keisuke Kodama, Hiroki Yamamoto, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses.
Biosci Biotechnol Biochem: 2005, 69(11);2155-69
[PubMed:16306698] [WorldCat.org] [DOI] (P p)

P Margot, V Lazarevic, D Karamata
Effect of the SinR protein on the expression of the Bacillus subtilis 168 lytABC operon.
Microb Drug Resist: 1996, 2(1);119-21
[PubMed:9158733] [WorldCat.org] [DOI] (P p)

V Lazarevic, P Margot, B Soldo, D Karamata
Sequencing and analysis of the Bacillus subtilis lytRABC divergon: a regulatory unit encompassing the structural genes of the N-acetylmuramoyl-L-alanine amidase and its modifier.
J Gen Microbiol: 1992, 138(9);1949-61
[PubMed:1357079] [WorldCat.org] [DOI] (P p)

A Kuroda, J Sekiguchi
Characterization of the Bacillus subtilis CwbA protein which stimulates cell wall lytic amidases.
FEMS Microbiol Lett: 1992, 74(1);109-13
[PubMed:1355454] [WorldCat.org] [DOI] (P p)