GcvH

• Description: glycine cleavage system protein H, 2-oxo acid dehydrogenase, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis
  
  

Gene name	gcvH
Synonyms	yusH
Essential	no
Product	glycine cleavage system protein H
Function	glycine utilization
Gene expression levels in SubtiExpress: gcvH
MW, pI	14 kDa, 3.888
Gene length, protein length	381 bp, 127 aa
Immediate neighbours	yusG, yusI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, Biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU32800

Database entries

• BsubCyc: BSU32800

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: gcvH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):
  • lipoic acid, lipoylated by LipM PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU32800

• Structure:

• UniProt: O32174

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: gcvH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Quin H Christensen, Natalia Martin, Maria C Mansilla, Diego de Mendoza, John E Cronan
A novel amidotransferase required for lipoic acid cofactor assembly in Bacillus subtilis.
Mol Microbiol: 2011, 80(2);350-63
[PubMed:21338421] [WorldCat.org] [DOI] (I p)

Quin H Christensen, John E Cronan
Lipoic acid synthesis: a new family of octanoyltransferases generally annotated as lipoate protein ligases.
Biochemistry: 2010, 49(46);10024-36
[PubMed:20882995] [WorldCat.org] [DOI] (I p)