pucB

pucB
168

xanthine dehydrogenase

Locus
BSU_32500
Molecular weight
20.91 kDa
Isoelectric point
7.92
Protein length
205 aaSequenceBlast
Gene length
618 bpSequenceBlast
Function
purine utilization
Product
xanthine dehydrogenase
Essential
no
Synonyms
pucB, yurE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2068 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, PucR regulon

Gene
Coordinates
3,339,331  3,339,948
The protein
Structure
2WAW (PDB) (from Mycobacterium tuberculosis, 27% identity)
O32146 (AlphaFold)
Expression and Regulation
Operons
Genes
pucA-pucB-pucC-pucD-pucE
Description
PubMed
Regulation
induced in the presence of purine nucleotides (effector: allantoin) (PucR) PubMed
Regulatory mechanism
PucR: activation, PubMed, in pucR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

pucA→pucE

2025-04-08 18:34:33

ghost

97

12efbe45279270e4577ccc311231d5d18e3f41c8

DEA144E8D38829EB38B1ABABEC21C2CCD65FEFF4

Biological materials
Mutant
MGNA-A941 (yurE::erm), available at the NBRP B. subtilis, Japan
BKE32500 (pucB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTCATACAACCGCCACCCG,  downstream forward: _UP4_TCTTGAACGGCCAAGTGACA
BKK32500 (pucB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTCATACAACCGCCACCCG,  downstream forward: _UP4_TCTTGAACGGCCAAGTGACA
References
Loading

99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11

Page visits: 1928

Time of last update: 2025-04-15 09:29:11

Author of last update: Jstuelk