DegQ

• Description: stimulates production of degradative enzymes and of extracellular poly-gamma-glutamate PubMed, stimulates phosphorylation of DegU by DegS PubMed, gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
  
  

Gene name	degQ
Synonyms	sacQ
Essential	no
Product	pleiotropic regulator
Function	regulation of exoenzyme synthesis
Gene expression levels in SubtiExpress: degQ
Interactions involving this protein in SubtInteract: DegQ
MW, pI	5 kDa, 6.536
Gene length, protein length	138 bp, 46 aa
Immediate neighbours	comQ, yuzC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation, transcription factors and their control

This gene is a member of the following regulons

ComA regulon, DegU regulon

The gene

Basic information

• Locus tag: BSU31720

Phenotypes of a mutant

loss of poly-gamma-glutamate production PubMed, no synthesis of nonribosomal peptides iturin A and plipastatin PubMed1 PubMed2, reduced synthesis of degradative enzymes PubMed, increased genetic competence PubMed

Database entries

• BsubCyc: BSU31720

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • stimulates autophosphorylation of DegS PubMed

• Protein family: degQ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DegQ-DegS PubMed

• Localization:

Database entries

• BsubCyc: BSU31720

• Structure:

• UniProt: Q99039

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: degQ PubMed

• Expression browser: degQ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by high cell density (ComA) PubMed
  • subject to carbon catabolite repression PubMed
  • controlled by DegU PubMed

• Regulatory mechanism:
  • ComA: transcription activation PubMed
  • DegU: transcription activation PubMed

• Additional information: the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed

Biological materials

• Mutant: QB4851 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed