InfC

• Description: translation initiation factor IF-3
  
  

Gene name	infC
Synonyms
Essential	yes PubMed
Product	initiation factor IF-3
Function	translation
Gene expression levels in SubtiExpress: infC
MW, pI	19 kDa, 10.342
Gene length, protein length	519 bp, 173 aa
Immediate neighbours	rpmI, yscB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

RplT regulon, stringent response

The gene

Basic information

• Locus tag: BSU28870

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU28870

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: IF-3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU28870

• Structure: 1TIF (N-terminal domain, Geobacillus stearothermophilus), 1TIG (C-terminal domain, Geobacillus stearothermophilus)

• UniProt: P55872

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: infC-rpmI-rplT-ysdA PubMed

• Expression browser: infC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:
  • binding of RplT to a RNA switch in the infC leader region causes transcription termination PubMed

• Additional information:
  • expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent processing PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Margaret M Elvekrog, Ruben L Gonzalez
Conformational selection of translation initiation factor 3 signals proper substrate selection.
Nat Struct Mol Biol: 2013, 20(5);628-33
[PubMed:23584454] [WorldCat.org] [DOI] (I p)

Patrice Bruscella, Karen Shahbabian, Soumaya Laalami, Harald Putzer
RNase Y is responsible for uncoupling the expression of translation factor IF3 from that of the ribosomal proteins L35 and L20 in Bacillus subtilis.
Mol Microbiol: 2011, 81(6);1526-41
[PubMed:21843271] [WorldCat.org] [DOI] (I p)

Ludek Sojka, Tomás Kouba, Ivan Barvík, Hana Sanderová, Zdenka Maderová, Jirí Jonák, Libor Krásny
Rapid changes in gene expression: DNA determinants of promoter regulation by the concentration of the transcription initiating NTP in Bacillus subtilis.
Nucleic Acids Res: 2011, 39(11);4598-611
[PubMed:21303765] [WorldCat.org] [DOI] (I p)

Nasslie Choonee, Sergine Even, Lena Zig, Harald Putzer
Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism.
Nucleic Acids Res: 2007, 35(5);1578-88
[PubMed:17289755] [WorldCat.org] [DOI] (I p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

V Biou, F Shu, V Ramakrishnan
X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix.
EMBO J: 1995, 14(16);4056-64
[PubMed:7664745] [WorldCat.org] [DOI] (P p)