mreD

168

the phenotype of mreD is similar to that of ''mreC

mreD is essential under normal conditions PubMed

Depletion of MreD leads to a progressive increase in the width and a decrease in the length of the cell and cells become lytic. In the depletion strain, lysis can be prevented and cell growth, but not cell shape, can be recovered by inculaion of Magnesium in the media.  This shape defect is consistent with a role for mreD in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation.

None/ structural

mreD family (single member, according to UniProt)

None

trans-membrane protein PubMed

during logarithmic growth, MreD forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis PubMed

forms transverse bands as cells enter the stationary phase PubMed

reports on helical structures formed by MreD PubMed seem to be misinterpretation of data PubMed

A conditional mutant with an inframe deletion of mreD complemented by a xylose inducible copy at an ectopic locus (strain named 4352) is avaliable from the Errington Group.

A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3416 PubMed.