pbpA

pbpA
168

class B penicillin-binding protein PBP 2A, required for cell wall synthesis during cell elongation, works together with RodA in the elongasome for lateral cell wall synthesis and control of cell diameter

Locus
BSU_25000
Molecular weight
79.96 kDa
Isoelectric point
9.57
Protein length
716 aaSequenceBlast
Gene length
2151 bpSequenceBlast
Function
formation of a rod-shaped peptidoglycan cell wall, spore outgrowth
Product
penicillin-binding protein PBP 2A
Essential
no
Synonyms
pbpA, yqgF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,581,771  2,583,921
Phenotypes of a mutant
a pbpA pbpH double mutant is not viable PubMed
The protein
Catalyzed reaction/ biological activity
required for cell wall synthesis during cell elongation PubMed
driver of MreB dynamics PubMed
transpeptidase activity PubMed
Protein family
transpeptidase family (according to UniProt)
Structure
3VSK (PDB) (from Staphylococcus aureus, 45% identity) PubMed
P54488 (AlphaFold)
Paralogous protein(s)
PbpH
Localization
extracellular (signal peptide) PubMed
during vegetative growth: evenly distributed along the membrane PubMed
localization depends on the availability of peptidoglyan precursors (lipid II) PubMed
Expression and Regulation
Operons
Genes
pbpA
Description
PubMed
Additional information
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
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pbpA

2025-03-21 03:40:28

ghost

98

9281f9b691db7f23f85efdefe838c9c794c501cf

6BC0F86B6B409DBC0C3E82261C20F656BBA6D28C

Biological materials
Mutant
MGNA-C461 (yqgF::erm), available at the NBRP B. subtilis, Japan
BKE25000 (pbpA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACATCACCTTTTCTAT,  downstream forward: _UP4_TAAAAAAAAACGCTCACATG
BKK25000 (pbpA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACATCACCTTTTCTAT,  downstream forward: _UP4_TAAAAAAAAACGCTCACATG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab
GFP fusion
3103  trpC2 pbpA::pSG5043 (cat Pxyl-gfpa-pbpA1-804) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC
Labs working on this gene/protein
Jeff Errington, Newcastle University, UK homepage
Original Publications
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Page visits: 6478

Time of last update: 2025-04-13 08:26:32

Author of last update: Jstuelk