AroB

• Description: 3-dehydroquinate synthase
  
  

Gene name	aroB
Synonyms
Essential	no
Product	3-dehydroquinate synthase
Function	biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroB
Metabolic function and regulation of this protein in SubtiPathways: aroB
MW, pI	40 kDa, 6.937
Gene length, protein length	1086 bp, 362 aa
Immediate neighbours	aroH, aroF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU22700

Database entries

• BsubCyc: BSU22700

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate (according to Swiss-Prot)

• Protein family: class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU22700

• Structure:

• UniProt: P31102

• KEGG entry: [2]

• E.C. number: 4.2.3.4

Expression and regulation

• Operon:
  • aroF-aroB-aroH (according to DBTBS)
  • aroF-aroB-aroH-trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE (according to DBTBS)

• Expression browser: aroB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A613 ( aroB::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

J A Hoch, E W Nester
Gene-enzyme relationships of aromatic acid biosynthesis in Bacillus subtilis.
J Bacteriol: 1973, 116(1);59-66
[PubMed:4200844] [WorldCat.org] [DOI] (P p)