DfrA

• Description: dihydrofolate reductase
  
  

Gene name	dfrA
Synonyms	tmp
Essential	yes PubMed
Product	dihydrofolate reductase
Function	biosynthesis of folate
Gene expression levels in SubtiExpress: dfrA
Interactions involving this protein in SubtInteract: DfrA
Metabolic function and regulation of this protein in SubtiPathways: dfrA
MW, pI	19 kDa, 5.219
Gene length, protein length	504 bp, 168 aa
Immediate neighbours	ypkP, thyB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU21810

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU21810

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH (according to Swiss-Prot)

• Protein family: spmB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DfrA-ThyB

• Localization:

Database entries

• BsubCyc: BSU21810

• Structure: 1ZDR (Geobacillus stearothermophilus)

• UniProt: P11045

• KEGG entry: [2]

• E.C. number: 1.5.1.3

Expression and regulation

• Operon: thyB-dfrA PubMed

• Expression browser: dfrA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kevin Francis, Vanja Stojkovic, Amnon Kohen
Preservation of protein dynamics in dihydrofolate reductase evolution.
J Biol Chem: 2013, 288(50);35961-8
[PubMed:24158440] [WorldCat.org] [DOI] (I p)

M Iwakura, M Kawata, K Tsuda, T Tanaka
Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon.
Gene: 1988, 64(1);9-20
[PubMed:2840350] [WorldCat.org] [DOI] (P p)

T T Myoda, V L Funanage
Coregulation of dihydrofolate reductase and thymidylate synthase B in bacillus subtilis.
Biochim Biophys Acta: 1985, 824(2);99-103
[PubMed:3918568] [WorldCat.org] [DOI] (P p)

T T Myoda, S V Lowther, V L Funanage, F E Young
Cloning and mapping of the dihydrofolate reductase gene of Bacillus subtilis.
Gene: 1984, 29(1-2);135-43
[PubMed:6436144] [WorldCat.org] [DOI] (P p)