yngJ

yngJ
168

3-methylbutanoyl-CoA dehydrogenase

Locus
BSU_18260
Molecular weight
40.76 kDa
Isoelectric point
5.79
Protein length
380 aaSequenceBlast
Gene length
1143 bpSequenceBlast
Function
mother cell metabolism, leucine utilization
Product
3-methylbutanoyl-CoA dehydrogenase
Essential
no
E.C.
1.3.99.10
Synonyms
yngJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1960 (Galperin et al., 2021)

This gene is a member of the following regulons

SigE regulon

Gene
Coordinates
1,956,218 → 1,957,360
The protein
Catalyzed reaction/ biological activity
3-methylbutanoyl-CoA  -→ 3-methylbut-2-enoyl-CoA PubMed
A + 2,3-saturated acyl-CoA --> 2,3-dehydroacyl-CoA + AH2 (according to UniProt)
Protein family
Acyl-CoA dehydrogenase family (according to UniProt)
Cofactors
FAD (according to UniProt)
Structure
3OWA (PDB) (from B. anthracis, 37% identity, 54% similarity)
O34421 (AlphaFold)
Paralogous protein(s)
MmgC, AcdA, FadE
Expression and Regulation
Operons
Genes
yngJ-yngI-yngH-yngHB-yngG-yngF-yngE
Description
PubMed
Regulation
expressed during sporulation in the mother cell (SigE) PubMed
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
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yngJ→yngE

2025-04-01 23:55:18

ghost

96

ec5ff58c486f073be542df6cbb7bc2ebb4e5deee

AC16F1F9497F11969688D82F5CC45D78083A44DD

Biological materials
Mutant
BKE18260 (ΔyngJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCGTTTCCTCCTTCAC,  downstream forward: _UP4_TAAAAATGTAAACGCTTTCC
BKK18260 (ΔyngJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCGTTTCCTCCTTCAC,  downstream forward: _UP4_TAAAAATGTAAACGCTTTCC
References
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Page visits: 2665

Time of last update: 2025-04-05 23:36:47

Author of last update: Melvin.boenninger