yngJ

yngJ
168

3-methylbutanoyl-CoA dehydrogenase

Locus
BSU_18260
Molecular weight
40.76 kDa
Isoelectric point
5.79
Protein length
380 aaSequenceBlast
Gene length
1143 bpSequenceBlast
Function
mother cell metabolism, leucine utilization
Product
3-methylbutanoyl-CoA dehydrogenase
Essential
no
E.C.
1.3.99.10
Synonyms
yngJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1960 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,956,218 → 1,957,360
The protein
Catalyzed reaction/ biological activity
3-methylbutanoyl-CoA  -→ 3-methylbut-2-enoyl-CoA PubMed
A + 2,3-saturated acyl-CoA --> 2,3-dehydroacyl-CoA + AH2 (according to UniProt)
Protein family
Acyl-CoA dehydrogenase family (according to UniProt)
Cofactors
FAD (according to UniProt)
Structure
3OWA (PDB) (from B. anthracis, 37% identity, 54% similarity)
O34421 (AlphaFold)
Paralogous protein(s)
MmgC, AcdA, FadE
Expression and Regulation
Operons
Genes
yngJ-yngI-yngH-yngHB-yngG-yngF-yngE
Description
PubMed
Regulation
expressed during sporulation in the mother cell (SigE) PubMed
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
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yngJ→yngE

2025-04-01 23:55:18

ghost

96

ec5ff58c486f073be542df6cbb7bc2ebb4e5deee

AC16F1F9497F11969688D82F5CC45D78083A44DD

Biological materials
Mutant
BKE18260 (ΔyngJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCGTTTCCTCCTTCAC,  downstream forward: _UP4_TAAAAATGTAAACGCTTTCC
BKK18260 (ΔyngJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCGTTTCCTCCTTCAC,  downstream forward: _UP4_TAAAAATGTAAACGCTTTCC
References
Hsiao TL, Revelles O, Chen L, Sauer U, Vitkup D Automatic policing of biochemical annotations using genomic correlations. Nature chemical biology. 2010 Jan; 6(1):34-40. doi:10.1038/nchembio.266. PMID:19935659
Steil L, Serrano M, Henriques AO, Völker U Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis. Microbiology (Reading, England). 2005 Feb; 151(Pt 2):399-420. . PMID:15699190
Eichenberger P, Jensen ST, Conlon EM, van Ooij C, Silvaggi J, González-Pastor JE, Fujita M, Ben-Yehuda S, Stragier P, Liu JS, Losick R The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis. Journal of molecular biology. 2003 Apr 11; 327(5):945-72. . PMID:12662922

BA28DFBAF88A4994B0541D1FF2187312FDE1FA15

Page visits: 2667

Time of last update: 2025-04-06 06:51:29

Author of last update: Melvin.boenninger