Difference between revisions of "CysH"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15570&redirect=T BSU15570]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysHP-sat-cysC-ylnDEF.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysHP-sat-cysC-ylnDEF.html]
Line 90: Line 91:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15570&redirect=T BSU15570]
  
 
* '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=2goy 2GOY] (from ''Pseudomonas aeruginosa'', 35% identity) {{PubMed|17010373}}
 
* '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=2goy 2GOY] (from ''Pseudomonas aeruginosa'', 35% identity) {{PubMed|17010373}}

Revision as of 13:40, 2 April 2014

  • Description: phosphoadenosine phosphosulfate sulfotransferase

Gene name cysH
Synonyms
Essential no
Product phosphoadenosine phosphosulfate sulfotransferase
Function sulfate reduction
Gene expression levels in SubtiExpress: cysH
Metabolic function and regulation of this protein in SubtiPathways:
cysH
MW, pI 26 kDa, 5.462
Gene length, protein length 699 bp, 233 aa
Immediate neighbours pyrE, cysP
Sequences Protein DNA DNA_with_flanks
Genetic context
CysH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysH expression.png















Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CymR regulon, S-box

The gene

Basic information

  • Locus tag: BSU15570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin (according to Swiss-Prot)
  • Protein family: CysH subfamily (according to Swiss-Prot)
  • Paralogous protein(s): YitB

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2GOY (from Pseudomonas aeruginosa, 35% identity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR)
    • induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism:
    • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
    • CymR: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References