guaD

guaD
168

guanine deaminase, general stress protein, important for survival of ethanol and paraquat stresses

Locus
BSU_13170
Molecular weight
17.01 kDa
Isoelectric point
5
Protein length
156 aaSequenceBlast
Gene length
471 bpSequenceBlast
Function
deamination of guanine to xanthine, purine salvage and interconversion
Product
guanine deaminase
Essential
no
E.C.
3.5.4.3
Synonyms
guaD, gde, yknA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0590 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,382,677 → 1,383,147
The protein
Catalyzed reaction/ biological activity
guanine + H+ + H2O --> NH4+ + xanthine (according to UniProt)
Protein family
Cytidine and deoxycytidylate deaminase family (according to UniProt)
Domains
CMP/dCMP-type deaminase domain (aa 1-132) (according to UniProt)
Structure
1WKQ (PDB)
O34598 (AlphaFold)
Expression and Regulation
Operons
Genes
guaD
Description
PubMed
Regulation
induced by stress (SigB) PubMed
Regulatory mechanism
PucR: activation, PubMed, in pucR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed, in sigB regulon
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guaD

2025-03-09 06:02:46

ghost

61

42512af819f887b390b78a35b593e9528ad64328

00E1BB001F9BFC50BC1BFED748FEFE960390C414

Biological materials
Mutant
MGNA-A752 (yknA::erm), available at the NBRP B. subtilis, Japan
BKE13170 (ΔguaD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCAATCCCTCCTGTCA,  downstream forward: _UP4_TAAAAGGATCAGGCATGCGC
BKK13170 (ΔguaD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCAATCCCTCCTGTCA,  downstream forward: _UP4_TAAAAGGATCAGGCATGCGC
References
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AEEC5CAC20D3A182074332087FD3CDCCF05F28CC

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Time of last update: 2025-04-12 11:11:56

Author of last update: TPed