xlyB
168

N-acetylmuramoyl-L-alanine amidase

Locus

BSU_12460

Molecular weight

33.71 kDa

Isoelectric point

9.8

Protein length

317 aaSequenceBlast

Gene length

954 bpSequenceBlast

Function

PBSX prophage-mediated lysis

Product

N-acetylmuramoyl-L-alanine amidase

Essential

E.C.

3.5.1.28

Synonyms

xlyB, yjpB

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

1,317,535 1,318,488

The protein

Catalyzed reaction/ biological activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)

Domains

contains a N-acetylglucosamine-polymer-binding LysM domain PubMed

contains an amidase_2 domain (like BlyA, CwlA, CwlH, XlyB)

N-acetylmuramoyl-L-alanine amidase domain (aa 40-142) (according to UniProt)

LysM domain (aa 177-221) (according to UniProt)

Structure

2L47 (PDB) (from Bacillus phage Gamma, corresponds to aa 3 ... 166, 57% identity)

O34391 (AlphaFold)

Paralogous protein(s)

CwlA, CwlH, XlyA

Localization

secreted (according to UniProt)

Expression and Regulation

Operons

Genes

xlyB

Description

PubMed

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Additional information

the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

BKE12460 (xlyB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT

BKK12460 (xlyB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTATCATCTCTCCTTTT, downstream forward: _UP4_TAAATGAAAAGACGGAAGCT

References

Reviews

Original Publications

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Time of last update: 2025-04-05 20:34:19

Author of last update: Jstuelk

xlyB 168

xlyB
168