iolX

iolX
168

scyllo-inositol 2-dehydrogenase

Locus
BSU_10850
Molecular weight
37.33 kDa
Isoelectric point
5.3
Protein length
342 aaSequenceBlast
Gene length
1029 bpSequenceBlast
Function
utilization of scyllo-inositol
Product
scyllo-inositol 2-dehydrogenase
Essential
no
Synonyms
iolX, yuxD, yucG, yisS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0673 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,164,370  1,165,398
Phenotypes of a mutant
impaired growth with scyllo-inositol as the only carbon source PubMed
The protein
Catalyzed reaction/ biological activity
NAD+ + scyllo-inositol --> H+ + NADH + scyllo-inosose (according to UniProt)
Protein family
Gfo/Idh/MocA family (according to UniProt)
Cofactors
NAD+ PubMed
Structure
3EZY (PDB) (from Thermotoga maritima, 37% identity)
P40332 (AlphaFold)
Paralogous protein(s)
YfiI, NtdC, YteT, IolU, IolG, YrbE, IolW
Expression and Regulation
Operons
Genes
iolX
Description
PubMed
Regulation
induced by scyllo-inositol PubMed
Regulatory mechanism
IolQ: repression, PubMed, in iolQ regulon
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iolX

2025-03-14 05:19:35

Jstuelk

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2323c37767d17c2578c469c1daee774c488632a7

063B871B7FF975AF6EB4CBCB262E200AA3D8B6AB

Biological materials
Mutant
MGNA-B191 (yisS::erm), available at the NBRP B. subtilis, Japan
BKE10850 (iolX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTCCCATCCTCTCCTTT,  downstream forward: _UP4_TAAAAGCTAAGTATGTTGCA
BKK10850 (iolX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTCCCATCCTCTCCTTT,  downstream forward: _UP4_TAAAAGCTAAGTATGTTGCA
References
Kurashiki R, Takahashi M, Okumura Y, Ono T, Endo H, Makino K, Fukui K, Yokoyama K, Ishikawa S, Yoshida K-i, Ohshiro T, Suzuki HEfficient pathway-driven scyllo-inositol production from myo-inositol using thermophilic cells and mesophilic inositol dehydrogenases: a novel strategy for pathway control.Applied and environmental microbiology. 2024 Jul 8; :e0028124. PMID: 38975762
Kang DM, Michon C, Morinaga T, Tanaka K, Takenaka S, Ishikawa S, Yoshida KI Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD(+)-dependent scyllo-inositol dehydrogenase. BMC microbiology. 2017 Jul 11; 17(1):154. doi:10.1186/s12866-017-1065-8. PMID:28693424
Morinaga T, Ashida H, Yoshida K Identification of two scyllo-inositol dehydrogenases in Bacillus subtilis. Microbiology (Reading, England). 2010 May; 156(Pt 5):1538-46. doi:10.1099/mic.0.037499-0. PMID:20133360

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Time of last update: 2025-04-10 14:49:32

Author of last update: Jstuelk