asnO

asnO
168

asparagine synthetase (glutamine-hydrolysing)

Locus
BSU_10790
Molecular weight
70.54 kDa
Isoelectric point
5.7
Protein length
614 aaSequenceBlast
Gene length
1845 bpSequenceBlast
Function
biosynthesis of asparagine
Product
asparagine synthetase (glutamine-hydrolysing)
Essential
no
E.C.
6.3.5.4
Synonyms
asnO, yisO, yucB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0367 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,157,237  1,159,081
Phenotypes of a mutant
inactivation of ''asnO'' renders cells asporogenous PubMed
The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-aspartate + L-glutamine --> AMP + diphosphate + H+ + L-asparagine + L-glutamate (according to UniProt)
Protein family
asparagine synthetase family (with AsnB and AsnH, according to UniProt)
Domains
Glutamine amidotransferase type-2 domain (aa 2-216) (according to UniProt)
Structure
1CT9 (PDB) (from E. coli, 27% identity) PubMed
O05272 (AlphaFold)
Expression and Regulation
Operons
Genes
asnO
Description
PubMed
Regulation
expressed during sporulation (SigE) PubMed
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
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asnO

2025-03-16 03:31:34

ghost

79

f36a9c45b4f022fec9018db991dd76ba0f1288f2

8FCD4A4A976908654802122E7AFB108380597A13

Biological materials
Mutant
GP4162 (asnO::kan), available in  Jörg Stülke's lab
BKE10790 (asnO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGACATCAGCTCCAAT,  downstream forward: _UP4_TAGGTTCAATACAAATGTGA
BKK10790 (asnO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGACATCAGCTCCAAT,  downstream forward: _UP4_TAGGTTCAATACAAATGTGA
References
Reviews
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Original Publications
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Page visits: 3519

Time of last update: 2025-04-08 08:30:44

Author of last update: Jstuelk