hlpB

hlpB
168

HNH nuclease-like protein, rescues AddA-AddB-mediated recombination intermediates

Locus
BSU_10660
Molecular weight
11.31 kDa
Isoelectric point
9.94
Protein length
100 aaSequenceBlast
Gene length
303 bpSequenceBlast
Function
rescuing of AddA-AddB-mediated recombination intermediates
Product
HNH nuclease-like protein
Essential
no
Synonyms
hlpB, yisB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
1,148,155  1,148,457
Phenotypes of a mutant
essential, the mutation can be suppressed by second-site mutations in addA or addB PubMed, non-essential according to PubMed
The protein
Structure
O06715 (AlphaFold)
Localization
evenly distributed throughout the cytosol in growing cells PubMed
forms a single focus on the nucleoid upon induction of DNA damage PubMed
Expression and Regulation
Operons
Genes
addB-addA-sbcD-sbcC-hlpB
Description
PubMed
Regulation
positive control by ComK PubMed
Regulatory mechanism
ComK: activation, in comK regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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addB→hlpB

2025-04-03 20:37:41

ghost

169

045c7bb396e5f5521fcc2041130afbaac29b0bd8

E5051999C31DBE142375FC331C2F21A76C29910D

Biological materials
Mutant
GP3559 (ΔhlpB::kan), available in Jörg Stülke's lab
MGNA-A503 (yisB::erm), available at the NBRP B. subtilis, Japan
BKE10660 (hlpB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC,  downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
BKK10660 (hlpB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC,  downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
References
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74E2AB77354355549D14152D3566C9B953A4CB31

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Time of last update: 2025-04-11 06:02:48

Author of last update: MBenda