rpsNB

rpsNB
168

alternative ribosomal protein S14*, can replace RpsN under conditions of zinc limitation

Locus
BSU_08880
Molecular weight
10.20 kDa
Isoelectric point
11.19
Protein length
89 aaSequenceBlast
Gene length
270 bpSequenceBlast
Function
translation
Product
alternative ribosomal protein S14*
Essential
no
Synonyms
rpsNB, rpsNB, yhzA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0199 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
965,909  966,178
The protein
Protein family
universal ribosomal protein uS14 family (with RpsN, according to UniProt)
Structure
5AFI (PDB) (the E. coli ribosome, 47% identity) PubMed
O31587 (AlphaFold)
Paralogous protein(s)
RpsN
Expression and Regulation
Operons
Genes
rpsNB
Description
PubMed
Regulation
induced by zinc starvation (Zur) PubMed
part of the third wave of induction upon zinc depletion PubMed
expression requires functional RnpM PubMed
Regulatory mechanism
Zur: repression, in the presence of zinc PubMed, in zur regulon
RnpM: unknown, PubMed, in rnpM regulon
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rpsNB

2025-04-04 12:18:41

Jstuelk

72

f6961f321e109e676dd476459beb0a3a1f6850ba

79B16E46120A3FA72B3E3C22EEA68AE4F36564E8

Additional information
the mRNA is very stable (> 15 min) PubMed
Biological materials
Mutant
BKE08880 (rpsNB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT,  downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC
BKK08880 (rpsNB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT,  downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC
References
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6212D2DB719ACC477DA835EAC198545318FFEFAC

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Time of last update: 2025-04-10 00:49:31

Author of last update: Melvin.boenninger