yfhO

yfhO
168

required for lipoteichoic acid glycosylation

Locus
BSU_08610
Molecular weight
93.56 kDa
Isoelectric point
9.63
Protein length
861 aaSequenceBlast
Gene length
2586 bpSequenceBlast
Function
lipoteichoic acid glycosylation
Product
glycosyltransferase
Essential
no
Synonyms
yfhO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4485 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
931,879 → 934,464
Phenotypes of a mutant
expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
The protein
Catalyzed reaction/ biological activity
moves GlcNAc sugar moieties from a C55-P intermediate onto the lipoteichoic acid backbone on the outside of the cell PubMed
Domains
13 predicted transmembrane helices and a large extracellular loop of 389 amino acids connecting the last two transmembrane helices PubMed
Structure
O31582 (AlphaFold)
Localization
cell membrane, with large extracellular loop PubMed
Expression and Regulation
Operons
Genes
csbB-yfhO
Description
PubMed
Regulation
induced by stress (SigB) PubMed
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
SigX: sigma factor, PubMed, in sigX regulon
Open in new tab

csbB→yfhO

2025-04-03 09:24:10

Jstuelk

89

0fc4613b5e5cb69bb108f0919877e1a50aa7004a

3B4BB64E9C67CF26512E14126535756D8A386B82

Biological materials
Mutant
MGNA-C320 (yfhO::erm), available at the NBRP B. subtilis, Japan
BKE08610 (ΔyfhO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGAATTCTCCAGTCTT,  downstream forward: _UP4_CTCGTCAGTCTGCTGCTTGC
BKK08610 (ΔyfhO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGAATTCTCCAGTCTT,  downstream forward: _UP4_CTCGTCAGTCTGCTGCTTGC
References
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BDFB6CCB5AAD9747971DE6B78E07558706E04617

Page visits: 2743

Time of last update: 2025-04-10 18:21:15

Author of last update: Jstuelk