csbB

csbB
168

lipoteichoic acid glycosyltransferase, generation of C55-P-Glc, general stress protein, required for protection against paraquate stress

Locus
BSU_08600
Molecular weight
37.57 kDa
Isoelectric point
9.2
Protein length
329 aaSequenceBlast
Gene length
990 bpSequenceBlast
Function
lipoteichoic acid glycosylation , survival of stress conditions
Product
lipoteichoic acid glycosyltransferase
Synonyms
csbB, yfhN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
930,818  931,807
The protein
Protein family
glycosyltransferase 2 family (according to UniProt)
Structure
5EKP (PDB) (from Synechocystis sp., 44% identity) PubMed
Q45539 (AlphaFold)
Paralogous protein(s)
YkoT, YkcC
Localization
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
csbB-yfhO
Description
PubMed
Regulation
induced by stress (SigB) PubMed
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
SigX: sigma factor, PubMed, in sigX regulon
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csbB→yfhO

2025-04-03 09:24:10

Jstuelk

89

0fc4613b5e5cb69bb108f0919877e1a50aa7004a

3B4BB64E9C67CF26512E14126535756D8A386B82

Biological materials
Mutant
MGNA-C319 (csbB::erm), available at the NBRP B. subtilis, Japan
BKE08600 (csbB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAGGCACCTTCTTTTT,  downstream forward: _UP4_TGAAAATACCAAGCGCCATT
BKK08600 (csbB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAAGGCACCTTCTTTTT,  downstream forward: _UP4_TGAAAATACCAAGCGCCATT
References
Rismondo J, Percy MG, Gründling A Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanisms for wall teichoic acid glycosylation. The Journal of biological chemistry. 2018 Mar 02; 293(9):3293-3306. doi:10.1074/jbc.RA117.001614. PMID:29343515
Percy MG, Karinou E, Webb AJ, Gründling A Identification of a Lipoteichoic Acid Glycosyltransferase Enzyme Reveals that GW-Domain-Containing Proteins Can Be Retained in the Cell Wall of Listeria monocytogenes in the Absence of Lipoteichoic Acid or Its Modifications. Journal of bacteriology. 2016 Aug 01; 198(15):2029-42. doi:10.1128/JB.00116-16. PMID:27185829
Ardiccioni C, Clarke OB, Tomasek D, Issa HA, von Alpen DC, Pond HL, Banerjee S, Rajashankar KR, Liu Q, Guan Z, Li C, Kloss B, Bruni R, Kloppmann E, Rost B, Manzini MC, Shapiro L, Mancia F Structure of the polyisoprenyl-phosphate glycosyltransferase GtrB and insights into the mechanism of catalysis. Nature communications. 2016 Jan 05; 7:10175. doi:10.1038/ncomms10175. PMID:26729507
Inoue H, Suzuki D, Asai K A putative bactoprenol glycosyltransferase, CsbB, in Bacillus subtilis activates SigM in the absence of co-transcribed YfhO. Biochemical and biophysical research communications. 2013 Jun 21; 436(1):6-11. doi:10.1016/j.bbrc.2013.04.064. pii:S0006-291X(13)00703-1. PMID:23632331
Reder A, Höper D, Gerth U, Hecker M Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis. Journal of bacteriology. 2012 Jul; 194(14):3601-10. doi:10.1128/JB.00528-12. PMID:22582280
Höper D, Völker U, Hecker M Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis. Journal of bacteriology. 2005 Apr; 187(8):2810-26. . PMID:15805528
Huang X, Helmann JD Identification of target promoters for the Bacillus subtilis sigma X factor using a consensus-directed search. Journal of molecular biology. 1998 May 29; 279(1):165-73. . PMID:9636707
Akbar S, Price CW Isolation and characterization of csbB, a gene controlled by Bacillus subtilis general stress transcription factor sigma B. Gene. 1996 Oct 24; 177(1-2):123-8. . PMID:8921856

824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C

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Time of last update: 2025-04-06 01:38:11

Author of last update: Jstuelk