hxlR

hxlR
168

MarR/DUF24 family transcription activator of the hxlA-hxlB operon

Locus
BSU_03470
Molecular weight
14.01 kDa
Isoelectric point
6.36
Protein length
120 aaSequenceBlast
Gene length
363 bpSequenceBlast
Function
regulation of the ribulose monophosphate pathway
Product
MarR/DUF24 family transcription activator
Essential
no
Synonyms
hxlR, yckH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1733 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
376,032 → 376,394
The protein
Catalyzed reaction/ biological activity
transcription activation of the ribulose monophosphate pathway for formaldehyde fixation and detoxification
Protein family
MarR/DUF24 family
Domains
HTH hxlR-type domain (aa 11-110) (according to UniProt)
Structure
7BZD (PDB) PubMed
P42406 (AlphaFold)
Effectors of protein activity
formaldehyde stimulates transcription activation by HxlR PubMed
Paralogous protein(s)
YdzF, HypR, YdeP, YtcD, YkvN, YodB
Expression and Regulation
Operons
Genes
hxlR
Description
PubMed
Additional information
A ncRNA is predicted between hxlR and srfAA PubMed
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hxlR

2025-04-07 16:30:18

Jstuelk

81

759626dda146eb8c61f729d9b95940275d51fc22

348EDDD2846B2315164A6B62ED1E91A6CAE629AA

Biological materials
Mutant
BKE03470 (ΔhxlR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATAGAATCCCCCCCCTTA,  downstream forward: _UP4_TAAGACGCTCTTCGCAAGGG
BKK03470 (ΔhxlR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATAGAATCCCCCCCCTTA,  downstream forward: _UP4_TAAGACGCTCTTCGCAAGGG
References
Zhu R, Zhang G, Jing M, Han Y, Li J, Zhao J, Li Y, Chen PRGenetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction.Nature communications. 2021 Jan 25; 12(1):581. PMID: 33495458
Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR. Nucleic acids research. 2012 May; 40(9):4178-92. doi:10.1093/nar/gkr1316. PMID:22238377
Irnov I, Sharma CM, Vogel J, Winkler WC Identification of regulatory RNAs in Bacillus subtilis. Nucleic acids research. 2010 Oct; 38(19):6637-51. doi:10.1093/nar/gkq454. PMID:20525796
Nguyen TT, Eiamphungporn W, Mäder U, Liebeke M, Lalk M, Hecker M, Helmann JD, Antelmann H Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR). Molecular microbiology. 2009 Feb; 71(4):876-94. doi:10.1111/j.1365-2958.2008.06568.x. PMID:19170879
Kato N, Yurimoto H, Thauer RK The physiological role of the ribulose monophosphate pathway in bacteria and archaea. Bioscience, biotechnology, and biochemistry. 2006 Jan; 70(1):10-21. . PMID:16428816
Yasueda H, Kawahara Y, Sugimoto S Bacillus subtilis yckG and yckF encode two key enzymes of the ribulose monophosphate pathway used by methylotrophs, and yckH is required for their expression. Journal of bacteriology. 1999 Dec; 181(23):7154-60. . PMID:10572115

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Time of last update: 2025-04-10 16:17:52

Author of last update: Jstuelk