Difference between revisions of "PutP"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03220&redirect=T BSU03220] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU03220&redirect=T BSU03220] | ||
* '''Structure:''' | * '''Structure:''' | ||
Revision as of 12:56, 2 April 2014
- Description: high affinity proline permease
| Gene name | putP |
| Synonyms | ycgO |
| Essential | no |
| Product | high affinity proline permease |
| Function | proline uptake |
| Gene expression levels in SubtiExpress: putP | |
| MW, pI | 48 kDa, 8.619 |
| Gene length, protein length | 1347 bp, 449 aa |
| Immediate neighbours | putC, putR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU03220
Phenotypes of a mutant
- no growth with proline as single source of carbon or nitrogen PubMed
Database entries
- BsubCyc: BSU03220
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- uptake of proline coupled to the uptake of sodium ions
- Protein family: sodium solute symporter family (SSS; TC2A.21)
- Paralogous protein(s): OpuE
Extended information on the protein
- Kinetic information:
- K(M) for proline: 8 +/- 2 µM PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU03220
- Structure:
- UniProt: P94392
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- available in Erhard Bremer's lab PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Adrienne Zaprasis, Tamara Hoffmann, Lorena Stannek, Katrin Gunka, Fabian M Commichau, Erhard Bremer
The γ-aminobutyrate permease GabP serves as the third proline transporter of Bacillus subtilis.
J Bacteriol: 2014, 196(3);515-26
[PubMed:24142252]
[WorldCat.org]
[DOI]
(I p)
Susanne Moses, Tatjana Sinner, Adrienne Zaprasis, Nadine Stöveken, Tamara Hoffmann, Boris R Belitsky, Abraham L Sonenshein, Erhard Bremer
Proline utilization by Bacillus subtilis: uptake and catabolism.
J Bacteriol: 2012, 194(4);745-58
[PubMed:22139509]
[WorldCat.org]
[DOI]
(I p)
Shih-Chien Huang, Ta-Hui Lin, Gwo-Chyuan Shaw
PrcR, a PucR-type transcriptional activator, is essential for proline utilization and mediates proline-responsive expression of the proline utilization operon putBCP in Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 12);3370-3377
[PubMed:21964733]
[WorldCat.org]
[DOI]
(I p)
Boris R Belitsky
Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon.
J Mol Biol: 2011, 413(2);321-36
[PubMed:21840319]
[WorldCat.org]
[DOI]
(I p)
