PutB

• Description: proline dehydrogenase (L-proline, NAD)
  
  

Gene name	putB
Synonyms	ycgM
Essential	no
Product	proline dehydrogenase (L-proline, NAD)
Function	proline utilization
Gene expression levels in SubtiExpress: putB
Metabolic function and regulation of this protein in SubtiPathways: putB
MW, pI	34 kDa, 7.291
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	ycgL, putC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, PutR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU03200

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

Database entries

• BsubCyc: BSU03200

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor (according to Swiss-Prot)

• Protein family: proline dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): FadM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU03200

• Structure:

• UniProt: P94390

• KEGG entry: [3]

• E.C. number: 1.5.99.8

Expression and regulation

• Operon: putB-putC-putP 22139509 PubMed

• Expression browser: putB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of proline (PutR) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • PutR: transcription activation PubMed
  • Spo0A: transcription activation PubMed
  • CodY: transcription repression, displacement of PutR PubMed

• Additional information:
  • overexpressed in a pnpA mutant PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Susanne Moses, Tatjana Sinner, Adrienne Zaprasis, Nadine Stöveken, Tamara Hoffmann, Boris R Belitsky, Abraham L Sonenshein, Erhard Bremer
Proline utilization by Bacillus subtilis: uptake and catabolism.
J Bacteriol: 2012, 194(4);745-58
[PubMed:22139509] [WorldCat.org] [DOI] (I p)

Shih-Chien Huang, Ta-Hui Lin, Gwo-Chyuan Shaw
PrcR, a PucR-type transcriptional activator, is essential for proline utilization and mediates proline-responsive expression of the proline utilization operon putBCP in Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 12);3370-3377
[PubMed:21964733] [WorldCat.org] [DOI] (I p)

Boris R Belitsky
Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon.
J Mol Biol: 2011, 413(2);321-36
[PubMed:21840319] [WorldCat.org] [DOI] (I p)

Gintaras Deikus, Paul Babitzke, David H Bechhofer
Recycling of a regulatory protein by degradation of the RNA to which it binds.
Proc Natl Acad Sci U S A: 2004, 101(9);2747-51
[PubMed:14976255] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)