opuAB

opuAB
168

glycine betaine and arsenobetaine ABC transporter (permease)

Locus
BSU_02990
Molecular weight
30.10 kDa
Isoelectric point
9.84
Protein length
282 aaSequenceBlast
Gene length
849 bpSequenceBlast
Function
compatible solute transport
Product
glycine betaine and arsenobetaine ABC transporter (permease)
Essential
no
Synonyms
opuAB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4176 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
322,271  323,119
Phenotypes of a mutant
inactivation of opuAB facilitates the adaptation a strain lacking c-di-AMP to growth on complex medium under anaerobic conditions PubMed
The protein
Catalyzed reaction/ biological activity
uptake of glycine betaine and arsenobetaine PubMed
uptake of dimethylglycine PubMed
Protein family
Binding-protein-dependent transport system permease family (according to UniProt)
CysTW subfamily (according to UniProt)
Domains
ABC transmembrane type-1 domain (aa 90-269) (according to UniProt)
Structure
P46921 (AlphaFold)
Localization
cell membrane PubMed
Expression and Regulation
Operons
Genes
opuAA-opuAB-opuAC
Description
PubMed
Regulation
induced by osmotic stress PubMed
Regulatory mechanism
RemA: activation, PubMed, in remA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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opuAA→opuAC

2025-04-04 06:52:03

ghost

116

eafb0a634093d79d1a26ed7a049acac8747a3e05

204CCFDF8D1DFA897A97ACEDDC7D4A3EDDBDB61A

Biological materials
Mutant
BKE02990 (opuAB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGGTATTCTAGGCAGTCTAT,  downstream forward: _UP4_AAGAAAAGCAGGGGGAATGC
BKK02990 (opuAB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGGTATTCTAGGCAGTCTAT,  downstream forward: _UP4_AAGAAAAGCAGGGGGAATGC
Expression vectors
pGP2925 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
Labs working on this gene/protein
Erhard Bremer, University of Marburg, Germany homepage
References
Reviews
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Original Publications
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5F989C57010ACFC03E37AF5A894153F432520921

Page visits: 2996

Time of last update: 2025-04-06 12:02:34

Author of last update: Jstuelk