Difference between revisions of "AnsZ"

From SubtiWiki
Jump to: navigation, search
Line 59: Line 59:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU02690&redirect=T BSU02690]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yccC.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yccC.html]
Line 96: Line 97:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU02690&redirect=T BSU02690]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1ZCF 1ZCF] (from ''Pectobacterium atrosepticum scri1043'', 55% identity, 73% similarity)
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1ZCF 1ZCF] (from ''Pectobacterium atrosepticum scri1043'', 55% identity, 73% similarity)

Revision as of 12:55, 2 April 2014

  • Description: asparaginase

Gene name ansZ
Synonyms yccC
Essential no
Product asparaginase
Function asparagine utilization
Gene expression levels in SubtiExpress: ansZ
Metabolic function and regulation of this protein in SubtiPathways:
ansZ
MW, pI 39 kDa, 6.15
Gene length, protein length 1125 bp, 375 aa
Immediate neighbours lmrA, lip
Sequences Protein DNA DNA_with_flanks
Genetic context
YccC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AnsZ expression.png




























Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU02690

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)
  • Protein family: asparaginase 1 family (according to Swiss-Prot)
  • Paralogous protein(s): AnsA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1ZCF (from Pectobacterium atrosepticum scri1043, 55% identity, 73% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References