mcsA

mcsA
168

regulatory subunit of the McsA-McsB arginine kinase holoenzyme, modulator of CtsR-dependent repression, McsA activates kinase activity of McsB

Locus
BSU_00840
Molecular weight
20.88 kDa
Isoelectric point
6.62
Protein length
185 aaSequenceBlast
Gene length
558 bpSequenceBlast
Function
control of CtsR activity
Product
activator of McsB kinase activity
Essential
no
Synonyms
mcsA, yacH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3880 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
101,927 → 102,484
The protein
Catalyzed reaction/ biological activity
binding to and activation of McsB arginine kinase activity PubMed
Domains
UVR domain (aa 139-174) (according to UniProt)
Structure
8WTC (PDB) McsB kinase domain complexed with McsA PubMed
8GQD (PDB) the McsA4-McsB4 complex of S. aureus PubMed
P37569 (AlphaFold)
Modification
phosphorylated on Arg-169 PubMed
Expression and Regulation
Operons
Genes
ctsR-mcsA-mcsB-clpC-radA-disA
Description
PubMed
Regulation
expressed during germination and spore outgrowth PubMed
Regulatory mechanism
CtsR: repression, PubMed, in ctsR regulon
Spx: activation, PubMed, in spx regulon
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed PubMed, in sigB regulon
SigF: sigma factor, PubMed, in sigF regulon
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ctsR→disA

2025-04-02 20:37:12

Bzhu

159

b7066b5ca5004b095502d71420c469e9ee925bfa

163769A1708F45F8ABE642374A97FA8A00293529

Biological materials
Mutant
MGNA-B929 (yacH::erm), available at the NBRP B. subtilis, Japan
mcsA::aphA3 available from the Gerth lab
BKE00840 (ΔmcsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT,  downstream forward: _UP4_GATAGTGAGGAGGAACAGGA
BKK00840 (ΔmcsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT,  downstream forward: _UP4_GATAGTGAGGAGGAACAGGA
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
Antibody
available in Gerth lab
References
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Time of last update: 2025-04-06 08:38:38

Author of last update: Jstuelk