rnmV

rnmV
168

RNase M5

Locus
BSU_00410
Molecular weight
20.53 kDa
Isoelectric point
6.68
Protein length
186 aaSequenceBlast
Gene length
561 bpSequenceBlast
Function
5S ribosomal RNA maturation
Product
endoribonuclease M5
Essential
no
Synonyms
rnmV, yabF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1658 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
50,087  50,647
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor (according to UniProt)
Protein family
Ribonuclease M5 family (single member, according to UniProt)
Domains
N-terminal TOPRIM domain (aa 4-94) PubMed
C-terminal NA-binding domain PubMed
Cofactors
two Mg2+ ions PubMed
Structure
6TPQ (PDB) (from Geobacillus stearothermophilus, 61% identity) PubMed
P37547 (AlphaFold)
Localization
cytoplasm (homogeneous) PubMed
Expression and Regulation
Operons
Genes
rnmV-ksgA
Description
PubMed
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rnmV→ksgA

2025-03-21 08:09:09

ghost

113

eed4866ffa4f654b46b2cc962cdb9f5b2d8e3935

9FAC2CB8F7E3367C2EFE6DC8D966C339F688D87B

Biological materials
Mutant
MGNA-B906 (yabF::erm), available at the NBRP B. subtilis, Japan
BKE00410 (rnmV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTTCCTCCAAGACAA,  downstream forward: _UP4_GATACCGTTATGCGGGAGGA
BKK00410 (rnmV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTTCCTCCAAGACAA,  downstream forward: _UP4_GATACCGTTATGCGGGAGGA
Labs working on this gene/protein
Ciaran Condon, IBPC, Paris, France Homepage
References
Reviews
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Original Publications
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5BA7AA691CFE4635DEFBD0B8B820F487B58A5DB7

Page visits: 3829

Time of last update: 2025-04-09 23:53:36

Author of last update: Jstuelk