Difference between revisions of "YabA"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00330&redirect=T BSU00330]
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00330&redirect=T BSU00330]
  
 
* '''Structure:'''
 
* '''Structure:'''

Revision as of 12:45, 2 April 2014

  • Description: inhibitor of DnaA oligomerization, tethers DnaA to the polymerase-clamp protein DnaN

Gene name yabA
Synonyms
Essential no
Product negative regulator of replication initiation
Function positioning of DnaA
Gene expression levels in SubtiExpress: yabA
Interactions involving this protein in SubtInteract: YabA
MW, pI 13 kDa, 6.065
Gene length, protein length 357 bp, 119 aa
Immediate neighbours yaaT, yabB
Sequences Protein DNA DNA_with_flanks
Genetic context
YabA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YabA expression.png















Categories containing this gene/protein

DNA replication

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00330

Phenotypes of a mutant

strongly reduced growth rate in minimal medium PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • negative control of replication initiation PubMed
    • associates with the oriC region in a DnaA-dependent manner, this limits the amount of DnaA that can bind the oriC region PubMed
    • YabA inhibits co-operative DNA binding by DnaA PubMed
    • inhibits oligomerization and helix formation of DnaA PubMed
  • Protein family: yabA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: leucine zipper
  • Modification:
  • Cofactor(s): Zn cluster
  • Effectors of protein activity:
    • DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Philippe Noirot, Jouy-en-Josas, France Homepage
Alan Grossman, Cambridge, MA, USA Homepage

Your additional remarks

References

Reviews

Original publications