Difference between revisions of "NasD"

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(Expression and regulation)
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''Cofactors:'''
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** Fe-S cluster  {{PubMed|11289299}}
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** FAD  {{PubMed|11289299}}
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''

Revision as of 15:29, 19 March 2014

  • Description: assimilatory nitrite reductase (subunit)

Gene name nasD
Synonyms nasBC, nirB
Essential no
Product assimilatory nitrite reductase (subunit)
Function utilization of nitrite as nitrogen source
Gene expression levels in SubtiExpress: nasD
Interactions involving this protein in SubtInteract: NasD
Metabolic function and regulation of this protein in SubtiPathways:
nasD
MW, pI 88 kDa, 4.948
Gene length, protein length 2415 bp, 805 aa
Immediate neighbours nasE, nasC
Sequences Protein DNA DNA_with_flanks
Genetic context
NasD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NasD expression.png















Categories containing this gene/protein

respiration, utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

NsrR regulon, ResD regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU03300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H (according to Swiss-Prot)
  • Protein family: nitrite and sulfite reductase 4Fe-4S domain family (according to Swiss-Prot)
  • Paralogous protein(s): NasB

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • nasD: expressed under anaerobic conditions (ResD) PubMed
    • induced by nitric oxide under anaerobic conditions (NsrR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original publications