NarK

• Description: nitrite extrusion protein
  
  

Gene name	narK
Synonyms
Essential	no
Product	nitrite extrusion protein
Function	nitrate respiration, nitrite export
Gene expression levels in SubtiExpress: narK
Metabolic function and regulation of this protein in SubtiPathways: narK
MW, pI	42 kDa, 10.099
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	fnr, argS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, respiration, membrane proteins

This gene is a member of the following regulons

Fnr regulon, NsrR regulon

The gene

Basic information

• Locus tag: BSU37320

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): NasA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: P46907

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: narK-fnr PubMed

• Expression browser: narK PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (Fnr) PubMed
  • induced by nitric oxide under anaerobic conditions (NsrR) PubMed

• Regulatory mechanism:
  • NsrR: transcription repression PubMed
  • Fnr: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

D J Richardson, B C Berks, D A Russell, S Spiro, C J Taylor
Functional, biochemical and genetic diversity of prokaryotic nitrate reductases.
Cell Mol Life Sci: 2001, 58(2);165-78
[PubMed:11289299] [WorldCat.org] [DOI] (P p)

Original publications

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414] [WorldCat.org] [DOI] (P p)

H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791] [WorldCat.org] [DOI] (P p)