Difference between revisions of "RecA"
Line 42: | Line 42: | ||
{{SubtiWiki category|[[DNA repair/ recombination]]}}, | {{SubtiWiki category|[[DNA repair/ recombination]]}}, | ||
{{SubtiWiki category|[[genetic competence]]}}, | {{SubtiWiki category|[[genetic competence]]}}, | ||
− | {{SubtiWiki category|[[phosphoproteins]]}} | + | {{SubtiWiki category|[[phosphoproteins]]}}, |
+ | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
Line 134: | Line 135: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
Line 163: | Line 165: | ||
<pubmed> 14527291 12045091 10506835 14616075 21517913 23046409 23380520 22933559 </pubmed> | <pubmed> 14527291 12045091 10506835 14616075 21517913 23046409 23380520 22933559 </pubmed> | ||
==Original publications== | ==Original publications== | ||
− | <pubmed>11814663,16061691,19060143,17803906,16024744,17630974,8226626,11555642, 16479537 19730681 7690748 17229847,16267290 20509597 20723756 17449621 21278288 22373918 22517742 23284295 23536821 21859751 23634894 23779106 24285298 24285298 24373815 </pubmed> | + | <pubmed>11814663,16061691,19060143,17803906,16024744,17630974,8226626,11555642, 16479537 19730681 7690748 17229847,16267290 20509597 20723756 17449621 21278288 22373918 22517742 23284295 23536821 21859751 23634894 23779106 24285298 24285298 24373815 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 16:52, 5 March 2014
- Description: multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
Gene name | recA |
Synonyms | recE |
Essential | no |
Product | multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage) |
Function | DNA repair/ recombination |
Gene expression levels in SubtiExpress: recA | |
Interactions involving this protein in SubtInteract: RecA | |
MW, pI | 37 kDa, 4.883 |
Gene length, protein length | 1041 bp, 347 aa |
Immediate neighbours | cinA, pbpX |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
DNA repair/ recombination, genetic competence, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16940
Phenotypes of a mutant
- drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
RecA filaments are dismantled from DNA by PcrA PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: recA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Effectors of protein activity:
Database entries
- Structure: 1U94 (RecA from E. coli, 62% identity, 86% similarity)
- UniProt: P16971
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: recA PubMed
- Regulation:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- IRN444 (cat), available in Jörg Stülke's lab
- 1A746 (recA::erm), PubMed, available at BGSC
- 1A786 (recA::kan), PubMed, available at BGSC
- BP469 (recA::erm), available in Fabian Commichau's lab
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Peter Graumann, Freiburg University, Germany homepage
Your additional remarks
References
Reviews
Juan C Alonso, Paula P Cardenas, Humberto Sanchez, James Hejna, Yuki Suzuki, Kunio Takeyasu
Early steps of double-strand break repair in Bacillus subtilis.
DNA Repair (Amst): 2013, 12(3);162-76
[PubMed:23380520]
[WorldCat.org]
[DOI]
(I p)
Dawit Kidane, Silvia Ayora, Joann B Sweasy, Peter L Graumann, Juan C Alonso
The cell pole: the site of cross talk between the DNA uptake and genetic recombination machinery.
Crit Rev Biochem Mol Biol: 2012, 47(6);531-55
[PubMed:23046409]
[WorldCat.org]
[DOI]
(I p)
Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559]
[WorldCat.org]
[DOI]
(I p)
Silvia Ayora, Begoña Carrasco, Paula P Cárdenas, Carolina E César, Cristina Cañas, Tribhuwan Yadav, Chiara Marchisone, Juan C Alonso
Double-strand break repair in bacteria: a view from Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(6);1055-81
[PubMed:21517913]
[WorldCat.org]
[DOI]
(I p)
Justin Courcelle, Philip C Hanawalt
RecA-dependent recovery of arrested DNA replication forks.
Annu Rev Genet: 2003, 37;611-46
[PubMed:14616075]
[WorldCat.org]
[DOI]
(P p)
Michael M Cox
The bacterial RecA protein as a motor protein.
Annu Rev Microbiol: 2003, 57;551-77
[PubMed:14527291]
[WorldCat.org]
[DOI]
(P p)
Shelley L Lusetti, Michael M Cox
The bacterial RecA protein and the recombinational DNA repair of stalled replication forks.
Annu Rev Biochem: 2002, 71;71-100
[PubMed:12045091]
[WorldCat.org]
[DOI]
(P p)
M M Cox
Recombinational DNA repair in bacteria and the RecA protein.
Prog Nucleic Acid Res Mol Biol: 1999, 63;311-66
[PubMed:10506835]
[WorldCat.org]
[DOI]
(P p)
Original publications