Difference between revisions of "Pgi"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[carbon core metabolism]]}}, | {{SubtiWiki category|[[carbon core metabolism]]}}, | ||
− | {{SubtiWiki category|[[phosphoproteins]]}} | + | {{SubtiWiki category|[[phosphoproteins]]}}, |
+ | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
− | <pubmed>17218307 19052382 4214896 23420519 11489127 4275311 11491085 17218307, 24571712 </pubmed> | + | <pubmed>17218307 19052382 4214896 23420519 11489127 4275311 11491085 17218307, 24571712 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 16:33, 5 March 2014
- Description: glucose 6-phosphate isomerase, glycolytic / gluconeogenic enzyme
Gene name | pgi |
Synonyms | yugL |
Essential | no |
Product | glucose-6-phosphate isomerase |
Function | enzyme in glycolysis / gluconeogenesis |
Gene expression levels in SubtiExpress: pgi | |
Metabolic function and regulation of this protein in SubtiPathways: pgi | |
MW, pI | 50.4 kDa, 4.85 |
Gene length, protein length | 1353 bp, 451 amino acids |
Immediate neighbours | yugM, yugK |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31350
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glucose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot) D-glucose 6-phosphate = D-fructose 6-phosphate
- Protein family: GPI family (according to Swiss-Prot) GPI family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification: phosphorylation on Thr-39 PubMed
- Effectors of protein activity: competitively inhibited by 6-phosphogluconate (in B.caldotenax, B.stearothermophilus) PubMed
- Localization:
- cytoplasm (according to Swiss-Prot), cytoplasm
Database entries
- UniProt: P80860
- KEGG entry: [3]
- E.C. number: 5.3.1.9
Additional information
- extensive information on the structure and enzymatic properties of Pgi can be found at Proteopedia
Expression and regulation
- Regulation: constitutively expressed PubMed
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant: GP508 (spc), available in Jörg Stülke's lab, PubMed
- Expression vector:
- pGP398 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- lacZ fusion: pGP510 (in pAC6), available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany homepage
Your additional remarks
References