Difference between revisions of "Icd"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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=== Extended information on the protein === | === Extended information on the protein === | ||
− | * '''Kinetic information:''' Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/4147570 PubMed] | + | * '''Kinetic information:''' |
+ | ** Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/4147570 PubMed] | ||
+ | ** specific activity: 1.64 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed|24571712}} | ||
− | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
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** ''in vitro'' phosphorylated by [[PrkC]] on Thr-138, Thr-147, and Thr-396 {{PubMed|20389117}} | ** ''in vitro'' phosphorylated by [[PrkC]] on Thr-138, Thr-147, and Thr-396 {{PubMed|20389117}} | ||
− | * ''' | + | * '''[[Cofactors]]:''' Mg<sup>2+</sup>, Mn<sup>2+</sup>, NADP<sup>+</sup> |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=icd_2979716_2980987_-1 icd] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=icd_2979716_2980987_-1 icd] {{PubMed|22383849}} | ||
− | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
** ''[[citZ]]'': [[SigA]] {{PubMed|8045899}} | ** ''[[citZ]]'': [[SigA]] {{PubMed|8045899}} | ||
** ''[[icd]]'': [[SigA]] {{PubMed|8045899}} | ** ''[[icd]]'': [[SigA]] {{PubMed|8045899}} | ||
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=References= | =References= | ||
− | <pubmed>10656796,12850135 11290745 10348849 11751849 18763711 17726680 16493705 4147570 20389117 9244258 20933603 22517742 24325460 </pubmed> | + | <pubmed>10656796,12850135 11290745 10348849 11751849 18763711 17726680 16493705 4147570 20389117 9244258 20933603 22517742 24325460 24571712</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 16:37, 28 February 2014
- Description: isocitrate dehydrogenase
Gene name | icd |
Synonyms | citC |
Essential | no |
Product | isocitrate dehydrogenase |
Function | TCA cycle |
Gene expression levels in SubtiExpress: icd | |
Interactions involving this protein in SubtInteract: Icd | |
Metabolic function and regulation of this protein in SubtiPathways: icd | |
MW, pI | 46 kDa, 4.833 |
Gene length, protein length | 1269 bp, 423 aa |
Immediate neighbours | mdh, citZ |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, membrane proteins, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29130
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH (according to Swiss-Prot)
- Protein family: isocitrate and isopropylmalate dehydrogenases family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Cofactors: Mg2+, Mn2+, NADP+
- Effectors of protein activity:
- Localization: attached to the membrane PubMed
Database entries
- Structure: 1HQS
- UniProt: P39126
- KEGG entry: [3]
- E.C. number: 1.1.1.42
Additional information
- This enzyme requires NADP+ exclusively. No activity was seen on the presence on NAD+ PubMed
- extensive information on the structure and enzymatic properties of Icd can be found at Proteopedia
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP666 (spc), GP672 (erm), available in Jörg Stülke's lab
- Expression vector:
- pGP1121 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody: available in Linc Sonenshein's lab
Labs working on this gene/protein
Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
Your additional remarks
References