Difference between revisions of "Icd"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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=== Extended information on the protein ===
 
=== Extended information on the protein ===
  
* '''Kinetic information:''' Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/4147570 PubMed]
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* '''Kinetic information:'''  
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** Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/4147570 PubMed]
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** specific activity: 1.64 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed|24571712}}
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''  
 
* '''Modification:'''  
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** ''in vitro'' phosphorylated by [[PrkC]] on Thr-138, Thr-147, and Thr-396 {{PubMed|20389117}}
 
** ''in vitro'' phosphorylated by [[PrkC]] on Thr-138, Thr-147, and Thr-396 {{PubMed|20389117}}
  
* '''Cofactor(s):''' Mg<sup>2+</sup>, Mn<sup>2+</sup>, NADP<sup>+</sup>
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* '''[[Cofactors]]:''' Mg<sup>2+</sup>, Mn<sup>2+</sup>, NADP<sup>+</sup>
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=icd_2979716_2980987_-1 icd] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=icd_2979716_2980987_-1 icd] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
 
** ''[[citZ]]'': [[SigA]] {{PubMed|8045899}}
 
** ''[[citZ]]'': [[SigA]] {{PubMed|8045899}}
 
** ''[[icd]]'': [[SigA]] {{PubMed|8045899}}
 
** ''[[icd]]'': [[SigA]] {{PubMed|8045899}}
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=References=
 
=References=
  
<pubmed>10656796,12850135 11290745 10348849 11751849 18763711 17726680 16493705 4147570 20389117 9244258 20933603 22517742 24325460 </pubmed>
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<pubmed>10656796,12850135 11290745 10348849 11751849 18763711 17726680 16493705 4147570 20389117 9244258 20933603 22517742 24325460 24571712</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:37, 28 February 2014

  • Description: isocitrate dehydrogenase

Gene name icd
Synonyms citC
Essential no
Product isocitrate dehydrogenase
Function TCA cycle
Gene expression levels in SubtiExpress: icd
Interactions involving this protein in SubtInteract: Icd
Metabolic function and regulation of this protein in SubtiPathways:
icd
MW, pI 46 kDa, 4.833
Gene length, protein length 1269 bp, 423 aa
Immediate neighbours mdh, citZ
Sequences Protein DNA DNA_with_flanks
Genetic context
Icd context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Icd expression.png















Categories containing this gene/protein

carbon core metabolism, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, CcpC regulon

The gene

Basic information

  • Locus tag: BSU29130

Phenotypes of a mutant

    • reduced ability to sporulate PubMed
    • growth and sporulation defects of the mutant could be partially bypassed by deletion of the major citrate synthase gene (citZ) PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH (according to Swiss-Prot)
  • Protein family: isocitrate and isopropylmalate dehydrogenases family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
    • Reversible Michaelis-Menten PubMed
    • specific activity: 1.64 µmol min-1 (mg protein)-1 PubMed
  • Modification:
  • Effectors of protein activity:
    • Inhibited by glyoxylate, oxaloacetate and oxalomalate PubMed
      • Better inhibition when glyoxylate and oxaloacetate is combined, probably due to the non-enzymatic conversion into oxalomalate, which is a strong inhibitor PubMed

Database entries

  • KEGG entry: [3]

Additional information

  • This enzyme requires NADP+ exclusively. No activity was seen on the presence on NAD+ PubMed
  • extensive information on the structure and enzymatic properties of Icd can be found at Proteopedia

Expression and regulation

  • Regulatory mechanism:
    • CcpA: transcription repression, CcpC: transcription repression PubMed
    • CcpC: transcription repression (molecular inducer: citrate) PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Your additional remarks

References